Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pharmaceutical application of lentivirus in regulating sulfydryl nitrosylation modification level of Septin2

A nitrosylation and horizontal technology, applied in gene therapy, antineoplastic drugs, drug combinations, etc., to achieve the effect of inhibiting the occurrence of aortic aneurysm and aortic dissection

Active Publication Date: 2021-07-09
NANJING MEDICAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether it can undergo sulfhydryl nitrosylation modification and its role and mechanism in cardiovascular diseases has not yet been studied or reported.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical application of lentivirus in regulating sulfydryl nitrosylation modification level of Septin2
  • Pharmaceutical application of lentivirus in regulating sulfydryl nitrosylation modification level of Septin2
  • Pharmaceutical application of lentivirus in regulating sulfydryl nitrosylation modification level of Septin2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Biotin-Switch method to detect thiol-nitrosylation-modified proteins (Cayman Chemical Company S-nitrosylation Protein Detection Kit, Cat. No. 10006518)

[0019] (1) After the cells are treated, remove the medium, wash three times with pre-cooled wash buffer, add 500 μL washbuffer to scrape, and transfer to a 1.5 mL centrifuge tube.

[0020] (2) Centrifuge at 500×g at 4°C for 5 min, and discard the supernatant.

[0021] (3) Place the cells on ice and configure the Blocking buffer. The specific preparation method is as follows: 100 μL of DMF and 900 μL of buffer A are added to the new blocking reagent to form the blocking buffer stock solution. After mixing, add 9 mL of buffer A, which is the Blocking buffer.

[0022] (4) Add 500 μL blocking buffer to each tube to resuspend the cell pellet obtained in (2), and rotate at 4°C for 30 min.

[0023] (5) Centrifuge at 12,000 rpm for 10 min at 4°C, and transfer the supernatant to a 15 mL centrifuge tube.

[0024] (6) Add 4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a pharmaceutical application of a lentivirus in regulating the sulfydryl nitrosation modification level of Septin2, belonging to the field of molecular biology diagnosis of aortic aneurysm and aortic dissection. According to the invention, the occurrence of the aortic aneurysm and the aortic dissection can be effectively inhibited by infecting the lentivirus into macrophages to down-regulate the sulfydryl nitrosation modification of the Cys111 site of the Septin2 protein.

Description

technical field [0001] The invention belongs to the field of molecular biology diagnosis of aortic aneurysm and aortic dissection, in particular to the pharmaceutical use of regulating the level of Septin2 sulfhydryl nitrosylation. Background technique [0002] Aortic aneurysm and aortic dissection (AAD) is a life-threatening disease with a high mortality rate in the event of aortic rupture. Over the past 20 years, major advances have been made in open surgery and endovascular repair of AAD. However, drug therapies are still lacking, therefore, the molecular and cellular mechanisms involved in the formation and development of AAD need to be explored. The main pathological features of AAD are the loss of vascular smooth muscle cells and the remodeling of the extracellular matrix due to the accumulation and activation of inflammatory cells. Inflammatory responses play a crucial role in AAD and largely influence many determinants of aortic wall remodeling. Macrophage-mediate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K48/00A61P9/00A61P35/00G01N27/62G01N33/574G01N33/68
CPCG01N33/6893G01N33/57407G01N33/57492G01N33/57496A61K48/0008A61K48/005A61P35/00A61P9/00G01N27/62G01N2800/329G01N2333/47
Inventor 季勇张昊张艳张旭红严科韩艺谢利平
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com