Micro-fluidic chip for accurately assembling single particles and single particle assembling method

Abstract

The invention discloses a micro-fluidic chip for accurately assembling a single particle and a single particle assembling method. The micro-fluidic chip structurally comprises a channel layer and a sealing layer, wherein the channel layer structurally comprises two parallel and independent channels, and each channel comprises a capture channel, an annular bypass and an assembly chamber. The method comprises the following operation steps: (1) capturing a single particle; (2) transferring the single particle to an assembly cavity by reversely introducing a solution; and (3) capturing and transferring multiple times to realize multi-cell assembly. According to the invention, tThe chip can achieve efficient single cell capture based on the fluid mechanics principle, assembly of single particles is controlled by controlling the flow direction of fluid, and accurate assembly of the single particles can be achieved through multiple times of repeated capture and assembly; and the micro-fluidic chip can be applied to the research and application fields of single-cell RNA sequencing, single-cell co-culture, cell secreted protein detection, single-cell interaction analysis and the like.

Description

technical field [0001] The invention relates to a microfluidic chip for precisely assembling single particles, which can be applied to fields such as single-cell RNA sequencing, single-cell co-cultivation, detection of cell secreted proteins, single-cell interaction analysis, and the like. Background technique [0002] The assembly of single particles is of great significance for studying the properties and functions of particles. Taking single cells as an example, cells have strong heterogeneity. Traditional analysis based on population cells often conceals the heterogeneity between cells and obtains the average expression Information and, in many cases, individualized single-cell functions play an important role in physiology as well as disease. Microfluidic chip is called "lab-on-a-chip", which has the advantages of miniaturization, integration, high throughput, size and single particle matching, so it is especially suitable for the analysis of single particle. However, ...

AI Technical Summary

Problems solved by technology

However, the controllable assembly capabilities of existing microfluidic chip devices are limited

For example, microarrays or droplet microfluidic systems are limited by the Poisson distribution, resulting in most micropores or droplets not containing single particles, and only a few containing single particles, which is even less accurate. Control the number of cells assembled in microwells or droplets

Most other types of microfluidic devices, such as microfluidic chips based on hydrodynamic trapping principles, still cannot achieve true precise assembly

In addition, in recent years, some microfluidic chips that can be used for the assembly of multiple particles have emerged. One operation can only achieve the capture and assembly of one type of single particle, and it is difficult to capture two different types of single particles at the same time in a single operation. and assemble

Images