Compound agent for promoting growth of potato seedlings and application thereof
A compounding agent, potato technology, applied in the field of microorganisms, can solve problems such as soil compaction in cultivated land
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[0029] In the present invention, the preparation method of the compound comprises the following steps:
[0030] (1) Mix Ningnan·Azoxystrobin with water to obtain a diluted medicament; the volume ratio of the Ningnan·Azoxystrobin suspension concentrate mixed with water is 0.84:674.16;
[0031] (2) mixing the Bacillus Velez HN-Q-8 fermented liquid with water to obtain a diluted bacterial agent; the volume ratio of the Bacillus Velez HN-Q-8 fermented liquid mixed with water is 450:225;
[0032] (3) After mixing the diluting medicament and the diluting bacterial agent, a compound agent is obtained;
[0033] Step (1) and step (2) are not limited in sequence.
[0034] The present invention does not have any limitation on the mixing methods of step (1), step (2) and step (3), and the methods well known to those skilled in the art can be adopted.
[0035] In the present invention, the mixed volume ratio of the diluted bacterial agent and the diluted medicament in step (3) is 3:7.
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Embodiment 1
[0043] The test medium includes LB medium and fermentation medium, wherein the fermentation medium formula: corn flour 20g / L, bean cake powder 20g / L, glucose 5g / L, peptone 2g / L, potassium dihydrogen phosphate 0.5g / L, Magnesium sulfate 0.05g / L, calcium carbonate 1g / L; LB medium: sodium chloride 10g / L, tryptone 10g / L, yeast extract 5g / L, agar 18g / L.
[0044] Test agent: 25% Ningnan·Azoxystrobin SC (Deqiang Biological Co., Ltd.); 45% Thifur·Azoxystrobin SC (Hebei Guanlong Agrochemical Co., Ltd.); Bacillus Velez HN- Q-8 fermentation broth.
Embodiment 2
[0048] Mix sterile vermiculite, soil and nutrient soil evenly in a mass ratio of 1:1:1. Put into sterilized flowerpots, cut the germinated seed potatoes into pieces and sow them. The diameter of the flowerpots is 17.5cm and the height is 14cm. The volume ratio of compound agent 1 (Ningnan·Azoxystrobin SC dilution agent and Bacillus velesi HN-Q-8 fermentation liquid is 7:3, and the number of viable bacteria is 3.6×10 8 cfu / mL), compound agent 2 (the volume ratio of Thiofur-Azoxystrobin SC and the fermentation broth of Bacillus velesi HN-Q-8 is 1:9, and the number of viable bacteria is 3.6×10 8 cfu / mL), compound agent 3 (the volume ratio of Thiofur-Azoxystrobin SC and the fermentation broth of Bacillus velesi HN-Q-8 is 2:8, and the number of viable bacteria is 3.6×10 8 cfu / mL), the dilute bacterial agent of the Bacillus monoschibeles HN-Q-8 fermentation broth (the number of viable bacteria is 3.6×10 8 cfu / mL), single application of 25% Ningnan · azoxystrobin suspension, single...
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