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Nanogold cone composite material with photo-thermal/photodynamic therapy performance and preparation method and application of nanogold cone composite material

A technology of photodynamic therapy and nano-gold cones, which is applied in photodynamic therapy, preparation of organic compounds, luminescent materials, etc., can solve the problems of no relevant reports in the field of antibacterial, and achieve the effect of improving targeting and efficient bactericidal function

Active Publication Date: 2021-06-29
WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an excellent thermotherapeutic agent, nano-gold cones are mainly used in anticancer at present, and there are no relevant reports on the application in the field of antibacterial

Method used

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  • Nanogold cone composite material with photo-thermal/photodynamic therapy performance and preparation method and application of nanogold cone composite material
  • Nanogold cone composite material with photo-thermal/photodynamic therapy performance and preparation method and application of nanogold cone composite material
  • Nanogold cone composite material with photo-thermal/photodynamic therapy performance and preparation method and application of nanogold cone composite material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The synthesis of embodiment 1 modified AIE photosensitizer

[0038] The synthetic route of modified AIE photosensitizer such as figure 1 As shown, it specifically includes the following steps:

[0039] (1) 4,4'-dihydroxybenzophenone and 1,4-dibromobutane to prepare compound 2:

[0040] 4,4'-dihydroxybenzophenone (10mmol), potassium carbonate (4mmol), and 1,4-dibromobutane (25mmol) were sequentially added into a 250mL round-necked flask, and refluxed for 12h. Cool down to room temperature, filter with suction, rinse the filter cake with absolute ethanol and water, and dry to obtain compound 2 (8.95 mmol), with a yield of 89.5%. Compound 2 has the chemical structure shown below:

[0041]

[0042] figure 2 It is the NMR spectrum of compound 2, and its hydrogen spectrum data are as follows: 1 H NMR (600MHz, CDCl 3 )δ7.78(d, J=8.7Hz, 4H), 6.95(d, J=8.7Hz, 4H), 4.08(t, J=6.0Hz, 4H), 3.51(t, J=6.6Hz, 4H) ,2.15(m,4H),2.05(m,4H).

[0043] (2) Compound 2 and 4-hydroxyb...

Embodiment 2

[0049] The synthesis of embodiment 2 modified nano-gold cones

[0050] (1) Preparation of nano-gold cones by seed crystal growth method:

[0051] In a 50mL centrifuge tube, add (9.5mL) H 2 O. (0.25mL) 0.01M sodium citrate solution, (0.1mL) 0.01M chloroauric acid solution, (0.15mL) 0.01M sodium borohydride solution, heated in a water bath at 30°C for 2h to prepare a seed solution. Add (10mL) 0.01M chloroauric acid solution, (2mL) 0.01M silver nitrate solution, (8mL) 1M hydrochloric acid solution to the cetyltrimethylammonium bromide solution with a concentration of 0.1mol / L, (2mL) ) 0.1M L-ascorbic acid solution to prepare culture medium. Finally, (7.5 mL) seed solution was added to (15 mL) culture solution, and stored at 28° C. for 5 hours until the color of the solution changed from clear to dark red.

[0052] (2) Nano gold cone purification:

[0053] The above solution was centrifuged for 10 min at a speed of 8000 rad / min. The obtained sediment was dispersed in (400mL) ...

Embodiment 3

[0056] The synthesis of embodiment 3 nano-gold cone composite material

[0057] Dissolve SH-PEG-COOH-modified gold nanoparticles (0.1 mmol) in pure water, and add (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.1 mmol ), N-hydroxysuccinimide (0.1mmol), after activation for half an hour, add modified AIE photosensitizer TPE-2As (0.1mmol), react for 12 hours, filter with suction, wash with water to get the target product, adopt transmission electron microscope The shape test was carried out on it, and the results were as follows: Image 6 shown.

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Abstract

The invention discloses a nanogold cone composite material with photo-thermal / photodynamic therapy performance and a preparation method and application thereof. The nanogold cone composite material is composed of a modified nanogold cone and a modified AIE photosensitizer connected to the surface of the modified nanogold cone, wherein the modified nanogold cone is a nanogold cone modified by sulfydryl and carboxyl groups; the modified AIE photosensitizer is an AIE photosensitizer connected with a quaternary ammonium cation; the modified AIE photosensitizer is designed into molecules with two quaternary ammonium salts, which are equivalent to two anchors and can better interact with negatively-charged bacterial membranes in bacteria, so the targeting property of a nanosystem is improved, and the nanosystem can realize the synergistic effect of photodynamic therapy and photothermal therapy on bacteria; and the AIE photosensitizer can also gather and induce luminescence imaging, so the nanogold cone composite material can be potentially applied to clinical bacterium diagnosis and treatment integrated research.

Description

technical field [0001] The invention relates to the technical field of new materials, in particular to a nano-gold cone composite material with photothermal / photodynamic therapy performance, a preparation method and application thereof. Background technique [0002] Bacterial infections have always been a serious threat to human health. Although the discovery of antibiotics has controlled bacterial infections, the widespread use and abuse of antibiotics has led to more and more bacteria developing drug resistance. The "super bacteria" Appearing is even worse. Therefore, overcoming the clinical problems caused by bacterial drug resistance and finding new antibacterial materials and antibacterial methods are current research hotspots in the field of antibacterial. [0003] Photodynamic therapy and photothermal therapy can effectively inhibit the emergence of bacterial drug resistance during the antibacterial process, and this unique advantage has attracted more and more atten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06C09K11/58C07C213/02C07C217/20C07C45/64C07C49/84C07C41/30C07C43/23A61K41/00A61K49/00A61P31/04
CPCA61K41/0052A61K41/0057A61K49/0021A61P31/04C07C41/30C07C45/64C07C213/02C09K11/02C09K11/06C09K11/58A61K2300/00C07C217/20C07C49/84C07C43/23
Inventor 王佰亮张衡瑞秦楠朱康宁高强褚晓莹曹小文杨梅
Owner WENZHOU INST UNIV OF CHINESE ACAD OF SCI
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