Microbial compound fermented tartary buckwheat enzyme and preparation method thereof
A technology of compound fermentation and tartary buckwheat enzyme, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of lack of official statistical data, difficult to guarantee product quality, exaggerated product efficacy, etc., and achieve a simple operation process. , The product performance is stable, and the effect of increasing the variety of applications
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Embodiment 1
[0021] A preparation method of tartary buckwheat ferment by microbial composite fermentation, comprising the following steps:
[0022] (1) Raw material preparation: crush tartary buckwheat with full fruit grains, no rot and mildew, through a 40-mesh sieve, and put it in a 4 ℃ refrigerator for later use;
[0023] (2) Strain activation: inoculate the yeast preserved on the slant into the wort medium, inoculate the Lactobacillus paracasei and Lactobacillus plantarum into the MRS solid medium, culture the yeast at 30°C, and culture the lactic acid bacteria at 37°C for 2 days;
[0024] (3) Seed solution culture: Weigh 40g of maltose syrup, fully dissolve it in a 1000ml beaker filled with 400ml of 75℃ water, divide it into three Erlenmeyer flasks, seal it with a parafilm, and sterilize it at 115℃ Sterilize in the pot for 20 minutes, after cooling to room temperature, insert the activated three strains into the aseptic operation table, label them, and culture them on a shaking table ...
Embodiment 2
[0032] A preparation method of tartary buckwheat ferment by microbial composite fermentation, comprising the following steps:
[0033] (1) Raw material preparation: crush tartary buckwheat with full fruit grains, no rot and mildew, through a 40-mesh sieve, and put it in a 4 ℃ refrigerator for later use;
[0034] (2) Strain activation: inoculate the yeast preserved on the slant into the wort medium, inoculate the Lactobacillus paracasei and Lactobacillus plantarum into the MRS solid medium, culture the yeast at 30°C, and culture the lactic acid bacteria at 37°C for 2 days;
[0035] (3) Seed solution culture: Weigh 45g of maltose syrup, fully dissolve it in a 1000ml beaker filled with 450ml of water at 75°C, divide it into three Erlenmeyer flasks, seal with a parafilm, and sterilize at 115°C Sterilize in the pot for 20 minutes, after cooling to room temperature, insert the activated three strains into the aseptic operation table, label them, and culture them on a shaking table f...
Embodiment 3
[0043] A preparation method of tartary buckwheat ferment by microbial composite fermentation, comprising the following steps:
[0044] (1) Raw material preparation: crush tartary buckwheat with full fruit grains, no rot and mildew, through a 40-mesh sieve, and put it in a 4 ℃ refrigerator for later use;
[0045] (2) Strain activation: inoculate the yeast preserved on the slant into the wort medium, inoculate the Lactobacillus paracasei and Lactobacillus plantarum into the MRS solid medium, culture the yeast at 30°C, and culture the lactic acid bacteria at 37°C for 2 days;
[0046] (3) Seed solution culture: Weigh 50g of maltose syrup, fully dissolve it in a 1000ml beaker filled with 500ml of water at 75°C, divide it into three Erlenmeyer flasks, seal with a parafilm, and sterilize at 115°C Sterilize in the pot for 20 minutes, after cooling to room temperature, insert the activated three strains into the aseptic operation table, label them, and culture them on a shaking table f...
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