Application of quercetin-3'-O-beta-D-glucoside as inhibitor of calcium ion channels

A technology of calcium ion channel and glucoside, which is applied in the application field of quercetin-3'-O-β-D-glucoside, and can solve problems such as no research shows

Active Publication Date: 2021-06-18
JIANGSU SUZHONG PHARM GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no research to show that quercetin-3′-O-β-D-glucoside inhibits TRPC channels, and in the preparation of TRPC channel-related cardiovascular diseases, coronary heart disease, atherosclerosis, advanced renal Use in medicine for exhaustion, neurological disease, chronic pain, acute pain or inflammatory disease

Method used

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  • Application of quercetin-3'-O-beta-D-glucoside as inhibitor of calcium ion channels
  • Application of quercetin-3'-O-beta-D-glucoside as inhibitor of calcium ion channels
  • Application of quercetin-3'-O-beta-D-glucoside as inhibitor of calcium ion channels

Examples

Experimental program
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Embodiment 1-6

[0074] (1) Transfect the cDNA plasmid vector containing TRPC3, TRPC6 or TRPC7 ion channels into the HERK293 cell line, and then select the corresponding antibiotics to incubate the cells according to the resistance of the plasmid to selectively screen the successfully transfected cells. Functional tests were performed on the surviving cells to confirm the expression and function of the channel protein, and then cloned and purified through the "limited dilution" process to obtain a stable cell line that stably expresses a specific channel.

[0075] (2) Add 10-13×104 cells / mL of the stable cells obtained in step (1), and add 150 μL of cell suspension per well into a 96-well plate with a black wall bottom. After 24 hours of cultivation in the incubator, it can be used for subsequent experiments.

[0076] (3) Observe the cells, and after confirming that the cells are in good condition, load the dye (fluo-4) for 60 min.

[0077] (4) prepare channel activator and inhibitor solution...

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Abstract

The invention relates to application of quercetin-3'-O-beta-D-glucoside as an inhibitor of calcium ion channels, and belongs to the field of medicine. The invention also provides application of quercetin-3'-O-beta-D-glucoside in preparation of drugs for treating cardiovascular diseases, coronary heart diseases, atherosclerosis, advanced renal failure, neurological diseases, chronic pain, acute pain or inflammatory diseases, and proves that quercetin-3'-O-beta-D-glucoside can inhibit calcium ion channels, especially TRPC ion channels, and the application of the quercetin-3'-O-beta-D-glucoside is expanded.

Description

technical field [0001] The technology of the invention belongs to the field of medicine, and in particular relates to the application of quercetin-3'-O-β-D-glucoside. Background technique [0002] Cardiovascular disease is the most harmful disease to human life and health in modern society. According to the report of the World Health Organization, about 17.9 million people died of cardiovascular disease in the world in 2016, accounting for 31% of the total global deaths. It can be seen that the medical needs of new drugs for the prevention and treatment of cardiovascular diseases are very urgent. Relevant studies have proved that TRPC channels are important pharmacological targets for the development of new drugs for some cardiovascular diseases such as cardiomyopathy, heart failure, hypertension and cerebrovascular diseases. Patent application WO2006 / 074802A1 discloses that TRPC channels are used in the treatment of cardiovascular and cerebrovascular diseases. Its research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61K36/185A61P9/00A61P9/10A61P13/12A61P25/00A61P29/00C12Q1/02
CPCA61K31/7048A61K36/185A61P9/00A61P9/10A61P13/12A61P25/00A61P29/00G01N33/5044C12N2503/02G01N2500/10
Inventor 唐海涛曹征宇余伯阳葛海涛王正俊种法政王殿广
Owner JIANGSU SUZHONG PHARM GRP CO LTD
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