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Strains and microbial inoculum for preventing and treating crop diseases as well as preparation method and application of strains and microbial inoculum

A crop disease and bacterial agent technology, applied in the field of bacterial agent and its preparation, to prevent and control crop disease strains, can solve the problems of soil, ecological environment hazards, unreasonable control measures, soil biodiversity reduction, etc., to reduce chemical pesticides and Use of fertilizers, improving the effect of soil micro-ecological environment, and promoting the healthy growth of crops

Active Publication Date: 2021-06-15
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, problems such as improper application of chemical fertilizers and pesticides, promotion of straw returning technology, and unreasonable control measures have caused the outbreak of various soil-borne and air-borne diseases of crops, seriously restricting the healthy development of the industry
Especially for some soil-borne diseases, due to continuous cropping obstacles, reduction of soil biodiversity, significant reduction of beneficial bacteria, complex pathogenic causes, and disease onset in the middle and late stages of plant growth or near the fruit harvesting period, it is difficult to use drugs in production. Specific Chemical Pesticides for Control
Although suitable chemical pesticides have a certain control effect, long-term application will cause pesticide residues, resistance of diseases and insect pests, and irreversible damage to soil and ecological environment; controlling the occurrence and damage of diseases through biological control is the current crop It is an effective way of green disease prevention and control. However, the current production of biocontrol agents for disease prevention and control has a single strain, and the number of effective viable bacteria has dropped significantly due to the interference of non-nutritional fillers and additives, and long shelf life during transportation. The field biocontrol effect is not ideal

Method used

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  • Strains and microbial inoculum for preventing and treating crop diseases as well as preparation method and application of strains and microbial inoculum
  • Strains and microbial inoculum for preventing and treating crop diseases as well as preparation method and application of strains and microbial inoculum
  • Strains and microbial inoculum for preventing and treating crop diseases as well as preparation method and application of strains and microbial inoculum

Examples

Experimental program
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Effect test

preparation example Construction

[0041] In a specific embodiment of the present invention, the preparation method of the fermentation liquid of Bacillus subtilis T122F preferably comprises: inoculating the Bacillus subtilis T122F bacterial suspension in the Bacillus subtilis liquid medium and carrying out shaking bed fermentation to obtain Bacillus subtilis T122F of fermentation broth. In the present invention, the shaker fermentation is preferably carried out in a sterile storage bottle; the inoculation amount of the Bacillus subtilis T122F bacterial suspension is preferably 0.1% to 0.5%, more preferably 0.2% to 0.4%, most preferably 0.25% to 0.35%; the ventilation rate of the shaker fermentation is preferably 80%; the temperature of the shaker fermentation is preferably 30 to 37°C, more preferably 32 to 36°C, most preferably 33 to 35°C; The time of the shaking table fermentation is preferably 38~48h, more preferably 40~46h, most preferably 42~44h; the rotation speed of the shaking table fermentation is pref...

Embodiment 1

[0064] Preparation of Bacillus subtilis T122F fermentation broth

[0065] Tested strain: Bacillus subtilis T122F Strain preservation number: CGMCC No 3043

[0066] The test medium includes Bacillus subtilis liquid medium; Bacillus subtilis liquid medium: corn flour 10g, soybean meal 20g, NaCl 5g, peptone 3g, yeast powder 5g, glucose 4g, calcium carbonate 2g and water 1000ml, pH is 6.5~ 7.0, ready for use after sterilization.

[0067]Preparation method: Inoculate the suspension of Bacillus subtilis bacteria into a sterile Erlenmeyer flask (80% ventilation rate) equipped with Bacillus subtilis liquid culture medium, the inoculum amount is 0.5%, and shake at a temperature of 30-37°C and 180-220rpm for 38 ~48h, obtain the fermented liquid of Bacillus subtilis, its effective live bacteria spore number is (10~100)×10 8 CFU / g.

Embodiment 2

[0069] Preparation of fermented product of Trichoderma longibrachiatum T202006

[0070] Trichoderma longibrachiata T202006 strain preservation number: strain preservation number CGMCC No.21462;

[0071] The test medium includes Trichoderma longibrachia liquid medium and Trichoderma longibrachia solid medium; Trichoderma longibrachia liquid medium: potato 200g, corn flour 10g, glucose 15g and water 1000mL, pH 6.5-7.0, sterilized For later use; Trichoderma longibrachiata solid medium: rice bran 600g, bran 400g, corn flour 25g, calcium carbonate 1g and water 200mL, mix well and sterilize for later use.

[0072] Preparation method: Trichoderma spore suspension is inoculated in a sterile Erlenmeyer flask (80% ventilation rate) equipped with Trichoderma longibrachiata liquid culture medium, and the inoculum size is 1%, and it is shaken and cultivated at a temperature of 25~28°C and 150~180rpm for 4~ 6d, obtain the fermented liquid of Trichoderma longibrati T202006; inoculate the Tr...

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Abstract

The invention relates to the field of disease prevention and control, in particular to a group of strains and microbial inoculum for preventing and controlling crop diseases as well as a preparation method and application of the strains and the microbial inoculum. The invention provides one group of strains for preventing and treating crop diseases, wherein the strains comprise bacillus subtilis T122F, trichoderma longibrachiatum T202006 and paecilomyces lilacinus; the preservation number of the bacillus subtilis T122F is CGMCC (China General Microbiological Culture Collection Center) No.3043; and the preservation number of the trichoderma longibrachiatum T202006 is CGMCC (China General Microbiological Culture Collection Center) No. 21462. The strains provided by the invention can effectively prevent and treat crop diseases, and are environment-friendly chemical pesticide and chemical fertilizer reduction use substitutes.

Description

technical field [0001] The invention relates to the field of disease prevention and control, in particular to a group of bacterial strains, bacterial agents, preparation methods and applications for preventing and controlling crop diseases. Background technique [0002] Plant diseases can be transmitted by soil, running water, airflow, seeds and insect vectors. Diseases transmitted by soil and running water are usually called soil-borne diseases, such as fusarium wilt, root rot, stem rot, root-knot nematode, etc., which are transmitted by airflow Diseases are usually called airborne diseases, such as fungal leaf spot, rice blast, gray mold, powdery mildew, etc. [0003] With the large-scale development of the planting industry, due to limited land resources, the area and duration of continuous cropping crops are also increasing year by year. In actual production, problems such as improper application of chemical fertilizers and pesticides, promotion of straw returning techn...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/38A01N63/30A01P21/00A01P3/00C05G3/60C12R1/125C12R1/885C12R1/79
CPCC12N1/20C12N1/14A01N63/22A01N63/38A01N63/30C05G3/60
Inventor 杨秀娟甘林卢学松代玉立兰成忠何玉仙
Owner INST OF PLANT PROTECTION FAAS
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