Novel compound extracted from burdock leaves and capable of protecting alcoholic liver injury as well as preparation method and application of novel compound

A technology of alcoholic liver injury and burdock leaf, which is applied in the field of medicine and can solve problems such as no public reports

Active Publication Date: 2021-06-11
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the compounds isolated from burdock leaves for regulating blood lipids, preventing and treating alcoholic l...

Method used

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  • Novel compound extracted from burdock leaves and capable of protecting alcoholic liver injury as well as preparation method and application of novel compound
  • Novel compound extracted from burdock leaves and capable of protecting alcoholic liver injury as well as preparation method and application of novel compound
  • Novel compound extracted from burdock leaves and capable of protecting alcoholic liver injury as well as preparation method and application of novel compound

Examples

Experimental program
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Embodiment 1

[0029] In the specific implementation of the present invention, a preparation method of a compound extracted from burdock leaves that protects alcoholic liver damage comprises the following steps:

[0030] (1) Grind 10 kg of dried burdock leaves, add water 9 times the weight of burdock leaves, soak at room temperature for 8 days, filter, and concentrate the filtrate to a concentrated solution (6 L) equivalent to 2 g / mL of crude drug;

[0031] (2) The concentrated solution is extracted 4 times sequentially with ethyl acetate, each dosage is 3L, 3L, 2L, 2L respectively, the ethyl acetate extracts are combined, and concentrated under reduced pressure to obtain the component Fr ETOAC (60g); component Fr ETOAC Carry out silica gel column chromatography with an inner diameter of 4 cm and a height of 20 cm, and elute with a dichloromethane-methanol gradient, the gradient ratio of dichloromethane-methanol is 100:3, and the dosage is 6L, and the gradient ratio of dichloromethane-methan...

Embodiment 2

[0035] In the specific implementation of the present invention, a preparation method of a compound extracted from burdock leaves that protects alcoholic liver damage comprises the following steps:

[0036] (1) 10 kg of dried burdock leaves are pulverized, added water with 8 times the weight of burdock leaves, soaked at room temperature for 11 days, filtered, and the filtrate is concentrated to a concentrated solution equivalent to crude drug 3g / mL;

[0037] (2) The concentrated solution was sequentially extracted 4 times with ethyl acetate, each dosage being 1.5L, 4.5L, 0.7L, and 2.8L respectively, the ethyl acetate extracts were combined, and concentrated under reduced pressure to obtain the component Fr ETOAC (20~40g); component Fr ETOAC Carry out silica gel column chromatography with an inner diameter of 4 cm and a height of 28 cm, and use dichloromethane-methanol gradient elution respectively, the gradient ratio of dichloromethane-methanol is 100:3, the dosage is 4L, the g...

Embodiment 3

[0041] In the specific implementation of the present invention, a preparation method of a compound extracted from burdock leaves that protects alcoholic liver damage comprises the following steps:

[0042] (1) 10 kg of dried burdock leaves are crushed, added with water 10 times the weight of burdock leaves, soaked at room temperature for 6 days, filtered, and the filtrate is concentrated to 5 L of concentrated solution equivalent to crude drug 0.5 g / mL;

[0043] (2) The concentrated solution was sequentially extracted 4 times with ethyl acetate, each dosage was 4.5L, 1.5L, 2.8L, 0.7L respectively, the ethyl acetate extracts were combined, and concentrated under reduced pressure to obtain the component Fr ETOAC (20~40g); component Fr ETOAC Carry out silica gel column chromatography with an inner diameter of 5.5 cm and a height of 10 cm, and elute with a dichloromethane-methanol gradient, the gradient ratio of dichloromethane-methanol is 100:3, the dosage is 9 L, and the gradien...

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Abstract

The invention relates to a novel compound extracted from burdock leaves and capable of protecting alcoholic liver injury as well as a preparation method and application of the novel compound, which can effectively solve the problem of preparing the novel compound capable of protecting alcoholic liver injury from burdock leaves and developing the novel application of the medicinal value of burdock leaves. The method comprises the following steps: soaking the burdock leaves in water, filtering, concentrating, sequentially extracting with ethyl acetate, concentrating an ethyl acetate extract under reduced pressure, carrying out silica gel column chromatography, carrying out gradient elution with dichloromethane-methanol, recovering a solvent under reduced pressure, then carrying out MCI column chromatography, carrying out gradient elution with a methanol aqueous solution, combining eluents, drying, and recrystallizing in methanol so as to obtain the novel compound extracted from burdock leaves and capable of protecting alcoholic liver injury. The preparation method is easy to operate, the guidance quality is high, the separation speed is high, the product purity is high, the compound can be effectively used for preparing the medicine for reducing the fat and protecting the alcoholic liver injury, the new application and the medicinal value of the burdock leaves are developed, and the economic and social benefits are huge.

Description

technical field [0001] The invention relates to medicine, especially a new compound extracted from burdock leaves and capable of protecting alcoholic liver damage, its preparation method and application. Background technique [0002] Burdock leaves are the dry basal leaves of the Compositae plant Arctiumlappa. They are widely distributed throughout the country. Among them, Shandong and Xuzhou are the main production areas. They have a long history of medicinal use. Burdock leaves have been recorded in ancient herbal medicines for preventing stroke and hand and foot paralysis. Folks still use burdock leaves to make tea and drink it to prevent hyperlipidemia. [0003] The incidence of alcoholic liver disease is increasing worldwide. It is a disease that develops progressively and will seriously endanger health and even life in the later stage. In addition to alcohol abstinence and symptomatic treatment, there is no effective treatment for alcoholic liver disease in modern med...

Claims

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Application Information

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IPC IPC(8): C07C35/36C07C29/74C07C29/76C07C29/86C07C29/78A61K31/047A61P1/16
CPCC07C35/36C07C29/74C07C29/76C07C29/86C07C29/78A61P1/16C07C2602/28C07B2200/07
Inventor 代丽萍梁韩晶冯庆梅朱利利龚曼池军张玲霞
Owner HENAN UNIV OF CHINESE MEDICINE
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