Brucella antibody rapid quantitative detection card and detection method

Abstract

The invention discloses a Brucella antibody rapid quantitative detection card and detection method. The Brucella antibody rapid quantitative detection card comprises a PVC bottom plate, a water absorption pad, a nitrocellulose membrane, a marker pad, a sample pad and a dilution pad which are installed in a shell. The nitrocellulose membrane is fixed to the PVC bottom plate, the left end of the nitrocellulose membrane is in lap joint with the water absorption pad, and the right end of the nitrocellulose membrane is in lap joint with the dilution pad; a marker pad is arranged at the right position of the middle part of the nitrocellulose membrane, a sample pad is arranged on the marker pad, and a quality control line and a detection line are arranged between the water absorption pad and the marker pad on the nitrocellulose membrane; the upper shell is provided with window holes corresponding to the detection line and the quality control line, one side of each window hole is provided with a quantitative identification line, a sample adding hole is formed corresponding to the sample pad, and a cleaning hole is formed corresponding to the dilution pad. According to the invention, the immune binding solution is accurately controlled by adding the sample into the sample adding hole, adding the diluent into the cleaning hole and arranging the quantitative marking line, so that not only can the detection sensitivity and precision be improved, but also the characteristics of rapidness, simplicity and convenience of the detection method are retained.

Description

technical field [0001] The invention relates to a detection card, in particular to a rapid quantitative detection card for Brucella antibody and a detection method. Background technique [0002] Brucellosis (brucellosis for short) is a highly contagious zoonotic disease caused by Brucella. The disease is transmitted through direct contact, digestive tract, respiratory tract and other ways. Brucellosis can seriously endanger human health, hinder the development of animal husbandry, and affect global economic trade. my country's law on the prevention and control of infectious diseases stipulates that brucellosis belongs to Class B infectious diseases and is listed as an occupational disease; my country's law on animal epidemic prevention stipulates that brucellosis is a second-class epidemic management infectious disease for animals, and sheep are Brucella between humans and animals main source of infection. In sheep brucellosis epidemic areas, the infection rate of the popu...

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Problems solved by technology

The enzyme label kit requires a microplate reader, incubation reaction conditions, multiple washes, etc. The detection linear range is narrow, and the requirements for the operating environment and professional technicians are high. In addition, the detection sample also requires complex pretreatment, such as blood collection. , separation of serum, etc.; and although rapid colloidal gold detection is convenient and does not require professional technicians and on-site detection, its detection sensitivity is low, and it can only be qualitative, and the detection linear range is narrow

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