Ion channel targeted microbubble as well as preparation method and application thereof

An ion channel and targeting technology, applied in the direction of pharmaceutical formulations, preparations for in vivo tests, medical preparations of non-active ingredients, etc., to achieve the effects of easy access, enhanced sensitivity, and improved sensory effects

Pending Publication Date: 2021-06-08
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of this application is to provide an ion channel targeting microbubble and its preparation method and its application, aiming to solve the problem that there is no targeting microbubble for ion channel at present

Method used

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  • Ion channel targeted microbubble as well as preparation method and application thereof
  • Ion channel targeted microbubble as well as preparation method and application thereof
  • Ion channel targeted microbubble as well as preparation method and application thereof

Examples

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preparation example Construction

[0055] The second aspect of the present application provides a method for preparing an ion channel targeting ultrasonic microbubble, including the following steps:

[0056] S01. Preparation of biometric microbubbles;

[0057] S02. Add biotinylated microbubbles to incubate, add biotin-labeled ion channel antibodies, incubation treatment, to obtain ultrasonic microbubbles to ion channels.

[0058] The new type of ionic channel provided by the present application is targeted by the preparation method of ultrasonic microbubbles, first preparing biotinized microbubbles, and then introduces affinity on the surface of biotinylated micrububbles, constructing biotin-affinity system The ion channel targeting ultrasonic microbubbles are prepared by biotin-affinity binding ion channel antibodies. The method is simple to operate, and the ion channel targets ultrasonic micropubbubs are easily obtained, more importantly, the resulting ion channel targets ultrasonic microbubbles, and can target t...

Embodiment 1

[0085] Preparation method of an ion channel targeting ultrasonic microbubble includes:

[0086] (1) 20 mg / ml of diaphragust phosphate choline (DSPC), 20 mg / ml of phosphate ester poly - ethylene glycol 2000 (DSPE-PEG2000) and 20 mg / ml of biological Plasmalated polyethylene glycol 2000 modified diaphragmy ester chloride (DSPE-PEG2000-Biotin) mixed in trichloromethane and mixed on the scroll mixer.

[0087] (2) The organic solvent was removed using a dry nitrogen to form a uniform film on the test tube wall, dried in a vacuum oven for 2-6 hours; then a volume-degassed Tris buffer solution was added to give a phospholipid solution.

[0088] (3) Heating the phospholipid solution to its phase transition temperature (55-60 ° C), and prepared a phospholipid solution with a water bath, dispensed into the small Western whipnee, and the air in the bottle is converted to perfluoropropane, and the mechanical oscillator is shocked. 45S gain biotinylated ultrasonic microbubbles.

[0089] (...

Embodiment 2

[0094] Piezo1 ion channel targeted microbubble sensitive ultrasound 2 Stimulating effect of A cells:

[0095] (1) Providing a Piezo1 ion channel targeting ultrasonic microbubble prepared by the above Example 1;

[0096] (2) Cultivate N 2 A cells are inoculated with 6-well plates, and when they are 75-85% cell density, use CA 2+ Fluorescent probe Fluo 4-AM labeled cells.

[0097] (3) Targeting the PIEZO1 ion channel of step (1) to incubate the cells of the ultrasonic microbubbles and the cells of step (2).

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Abstract

The invention relates to the technical field of ultrasonic nerve stimulation, and provides an ion channel targeted microbubble as well as a preparation method and application thereof. The ion channel targeted ultrasonic microbubble comprises a shell membrane and a biological inert gas, the biological inert gas is sealed in a capsule enclosed by the shell membrane, and the outer surface of the shell membrane is combined with an ion channel antibody. According to the ion channel targeted microbubble provided by the invention, through targeted binding of the ion channel antibody and the ion channel, ultrasonic energy for opening the ion channel by ultrasonic radiation force can be reduced, and extracellular ions are promoted to flow into cells, so that the sensitivity of ultrasonic nerve stimulation response is enhanced.

Description

Technical field [0001] The present application belongs to the field of ultrasound nerve stimulation, and more particularly to an ionic channel targeted microbubble and its preparation method and application thereof. Background technique [0002] In recent years, nerve regulation techniques (such as electricity, magnetic and optical genetic technology, etc.) based on the physics are become an important driving force for rapid development of moving nerve, and provides new directions for treating brain diseases. Ultrasound-based non-invasive neurological regulation technology is considered to be one of the next generation of neurological regulation techniques with the most clinically transformed prospects, which has the advantages of non-invasive, large penetration depth, high-temporal distribution rate. Ultrasound nerve regulation passes through different intensity, frequency, pulse repetitive pulse width, duration causes the central nerve of the stimulus to generate two-way regula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K47/68A61K47/55A61P25/00A61P25/14A61P25/08A61P25/16A61P25/24A61P25/30A61P25/20
CPCA61K49/223A61K49/221A61K47/68A61K47/557A61P25/00A61P25/14A61P25/08A61P25/16A61P25/24A61P25/30A61P25/20
Inventor 严飞谢丽婷
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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