Online detection method for content of short-chain fatty acid in panda excrement based on gas chromatography

A short-chain fatty acid and gas chromatography technology, applied in the field of short-chain fatty acid detection, can solve the problems of high derivatization operation requirements, time-consuming reagent storage, poor safety, etc., and achieve high detection sensitivity, fast detection speed and repeatability Good results

Pending Publication Date: 2021-06-01
CHENGDU RES BASE OF GIANT PANDA BREEDING
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Problems solved by technology

[0004] The present invention aims to provide an online detection method for short-chain fatty acid content in giant panda feces based on gas chromatography to solve the technical problems of poor safety, high requirements for derivatization operations, time-consuming and troublesome storage of reagents used in the prior art

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  • Online detection method for content of short-chain fatty acid in panda excrement based on gas chromatography
  • Online detection method for content of short-chain fatty acid in panda excrement based on gas chromatography

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Embodiment 1

[0022] The online detection method for the content of short-chain fatty acids in giant panda feces provided by this embodiment comprises the following steps:

[0023] 1. Solution preparation

[0024] A total of 7 kinds of acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, and hexanoic acid were prepared from the SCFAs mixed standard stock solution, and a certain volume of each standard was drawn according to the density, diluted with 20% phosphoric acid, and prepared as 10mg / mL mixed standard stock solution, then diluted with 20% phosphoric acid to 1mg / mL mixed standard stock solution. Then use 20% phosphoric acid to dilute step by step, configure a mixed standard solution, and refrigerate at 4°C for later use.

[0025] The internal standard stock solution was prepared with 2-ethylbutyric acid as the internal standard, and the 2-ethylbutyric acid was absorbed and diluted with 20% phosphoric acid to prepare a 10 mg / mL internal standard s...

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Abstract

The invention discloses an on-line detection method for the content of short-chain fatty acid in panda excrement based on gas chromatography, and belongs to the technical field of short-chain fatty acid detection. The method comprises the steps that: 2-ethyl butyric acid serves as an internal standard substance, a standard substance solution is prepared, the internal standard substance solution is added according to the standard solution to be detected on a machine, and a standard curve of the peak area and the solution concentration is prepared, and a standard curve equation is obtained; and the internal standard substance solution is added into a sample solution, detection is performed on the machine to obtain a map; and calculation is performed according to the standard curve equation to obtain the content of a corresponding target object. The detection method provided by the invention is safe and rapid in extraction, high in detection sensitivity, good in repeatability and high in detection speed, can be used for batch treatment, can be used for efficient and accurate quantification, and has good scientific research prospects and popularization significance.

Description

technical field [0001] The invention relates to the technical field of detection of short-chain fatty acids, in particular to an online detection method for the content of short-chain fatty acids in giant panda feces based on gas chromatography. Background technique [0002] Short Chain Fatty Acids (Short Chain Fatty Acids, referred to as SCFA) are volatile organic acids with 1 to 6 carbon atoms, which are produced by the metabolism of intestinal flora, mainly including acetic acid, propionic acid and butyric acid. It can provide energy for intestinal epithelial cells and play an important role in maintaining intestinal integrity and regulating intestinal immune function. Under enteritis conditions, SCAFs promote the proliferation of intestinal mucosal cells, protect the intestinal barrier, and inhibit the secretion of pro-inflammatory factors to relieve intestinal inflammation. Different short-chain fatty acids can reflect the types of intestinal flora of giant pandas, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 张文平郑丽君谢军金侯蓉王海瑞张志和黄河陈鹏张亮沈富军刘佳文
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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