Composition for treating liver cancer and its application

A composition and a technology for inhibiting liver cancer, applied in the field of biomedicine, can solve problems such as poor prognosis, achieve the effects of less toxic side effects and inhibiting tumor growth

Active Publication Date: 2022-06-21
CANCER CENT OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, dox resistance often occurs in advanced tumors, resulting in poor prognosis (Chen et al., 2018)

Method used

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  • Composition for treating liver cancer and its application
  • Composition for treating liver cancer and its application
  • Composition for treating liver cancer and its application

Examples

Experimental program
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Effect test

experiment example 1

[0041] 1. Cell culture, passage and cryopreservation

[0042] (1) Preparation of cell culture-related reagents and cell culture:

[0043] 1X PBS preparation (1L): Weigh 8g NaCl, 0.2g KCl, 3.58g Na 2 HPO 4 ·12H 2 O 2 ,0.24g KH 2 PO 4 , Dissolve in 800ml of deionized water, stir well, and dilute to 1L with a graduated cylinder. Use after autoclaving.

[0044] 0.25% pancreatin preparation (0.02% EDTA) (1L): add 8g NaCl, 0.4g KCl, 0.12g Na in about 800ml ultrapure water 2 HPO 4 ·12H 2 O 2 ,0.06g KH 2 PO 4 ,0.35g NaHCO 3 , 1g glucose, 0.01g phenol red, 2.5g pancreatin, then add 1.07ml 0.5mol / L EDTA, stir well to dissolve, dilute to 1L, and adjust pH to 7.2.

[0045] DMEM basal culture preparation (1L): Dissolve a packet of DMEM powder (Gibco, 12100-046) in about 800mL of ultrapure water, stir well with a magnetic stirrer for 2 hours, and weigh 3g HEPES and 3.17g NaHCO respectively. 3 Add it to it, and when it is completely dissolved, make up the volume to 1L.

[004...

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Abstract

The invention belongs to the field of biomedicine and specifically discloses that Crizo combined with Dox synergistically induces cancer cell death. Compared with single-drug treatment, the combination of the two drugs increased cell death by at least 50%, which was much stronger than the combination of sorafenib and Dox, the first-line treatment drugs for advanced liver cancer, in inducing apoptosis of liver cancer cells. The present invention clarifies the mechanism of the above effects from the cellular level, and reveals that Crizo combined with Dox can overcome the resistance to chemotherapeutic drugs by inhibiting the expression of MDR1 and inducing autophagic cell death. Combined with in vivo experiments, it shows that Crizo combined with Dox can effectively inhibit tumor growth in animal models with less toxic side effects, which provides a potential therapeutic strategy for the treatment of cancer, especially advanced liver cancer.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, more particularly, to a composition for treating liver cancer, specifically a pharmaceutical composition containing crizotinib and doxorubicin and its application in the preparation of a drug for treating liver cancer. Background technique [0002] Liver cancer is the sixth most common cancer worldwide and the third leading cause of cancer-related death. According to the risk factors for liver cancer, the incidence and mortality of liver cancer were significantly associated with age, sex, and geographic region. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic ductal carcinoma and other rare types. Among them, hepatocellular carcinoma with the highest degree of malignancy accounts for more than 80% and is the main cause of death in patients with liver cancer. Surgical resection and liver transplantation are the most effective treatments for hepatocellular car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4545A61K31/704A61P1/16A61P35/00
CPCA61K31/4545A61K31/704A61P1/16A61P35/00A61K2300/00
Inventor 李朝阳张必翔
Owner CANCER CENT OF GUANGZHOU MEDICAL UNIV
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