Lycium barbarum polysaccharide with hypolipidemic effect
A technology of Lycium barbarum polysaccharide and Lycium barbarum, which is applied in the field of pharmaceutical chemistry, can solve the problems of unclear material basis and structure of hypolipidemic polysaccharide, wide molecular weight distribution, etc.
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[0026] Example 1: Preparation of Lycium barbarum homogeneous polysaccharide LBP009-2-1 with hypolipidemic effect
[0027] 1. Preparation method of Lycium barbarum homogeneous polysaccharide LBP009-2-1
[0028] 1.1 Instruments, reagents and materials
[0029] DEAE-52 cellulose, Sephadex G-50, standard monosaccharides (arabinose, ribose, xylose, glucose, fructose, mannose, galactose, fucose, rhamnose, galactobionic acid, glucuronic acid and mannose sugar acid). All other chemicals were at least of analytical grade. The solvent for HPLC is chromatographically pure.
[0030] 1.2 Preparation of Lycium barbarum crude polysaccharide
[0031] figure 1 It is a flow chart of separating and purifying Lycium barbarum homogeneous polysaccharide LBP009-2-1 from Lycium barbarum.
[0032] Add 1L of distilled water to 100g of wolfberry, and use a high-speed shear dispersing and emulsifying machine to assist extraction, with a rotation speed of 15,000 rpm and an extraction temperature of ...
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