Microscopic imaging method and system based on double-core optical fiber light control and dynamic speckle illumination

Abstract

The invention provides a microscopic imaging method and system based on double-core optical fiber light control and dynamic speckle illumination. The method is characterized in that a light control part of the device is formed by oppositely installing two two-core optical fibers of which the output end surfaces are processed into specific angles. Laser beams are respectively coupled into a two-core optical fiber through a single-mode optical fiber, a focusing light field is formed near an output end face, and cells to be detected are stably captured. And the cells rotate around a specific axis by adjusting the output power of each fiber core of the other two-core optical fiber. And after the cells rotate to a certain angle and reach a stable state every time, a tomographic image of the cells is acquired by using a dynamic speckle illumination wide-field fluorescence microscopy technology, and finally a three-dimensional structure image of the cells is reconstructed. The system constructed by the invention can be used for obtaining a three-dimensional structure image with high temporal and spatial resolution of a living single cell, has the characteristics of simple structure, low cost, simplicity and convenience in operation and the like, and has wide application prospects in numerous research fields such as biology, medicine, life science and the like.

Description

[0001] (1) Technical field [0002] The invention relates to a microscopic imaging method and system based on dual-core fiber optic manipulation and dynamic speckle illumination, which can be used for high-time-resolution and high-space-resolution imaging of living single-cell three-dimensional structures, and belongs to the field of biophotonics. [0003] (2) Background technology [0004] Cells are the basic unit of life structure and function. In-depth research on cells is the key to revealing the mysteries of life phenomena, transforming life and overcoming diseases. By analyzing single cells, it is possible to study phenomena and mechanisms that cannot be observed in large populations of cells. On the one hand, there are different levels of life systems. In order to study the life systems of different levels and their interrelationships, the first thing to study in biological science is cells. On the other hand, most of the reactions in organisms are carried out in cells....

AI Technical Summary

Problems solved by technology

The traditional optical tweezers system needs to use a large numerical aperture objective lens to form a strong optical potential well at the focal point to achieve cell capture and manipulation. The system is bulky, has poor flexibility, is expensive, and is subject to many factors during use, such as and substrate compatibility limitations

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