Serum amyloid protein A calibrator diluent as well as preparation method and application thereof

Abstract

The invention relates to a serum amyloid protein A calibrator diluent and application thereof. The diluent comprises a protein protective agent and a preservative, the protein protective agent is at least one of carbohydrates, protein substances, electrolyte, alcohols and a surfactant, and the carbohydrates are selected from at least one of deoxyribose, proteoglycan, erythritol and quinoa polysaccharide. The diluent can effectively remove the calibrator diluent, is helpful for improving the detection accuracy, can implement a liquid transportation mode, and reduces the unnecessary freeze-drying process of calibrator dilution. The calibrator provided by the embodiment of the invention is also applied to daily reagent evaluation, and has no interference effect on evaluation work of precision, sensitivity, detection limit, accuracy and the like.

Description

technical field [0001] The invention relates to the technical field of biochemical detection reagents, in particular to a serum amyloid A calibrator dilution, a preparation method and application thereof. Background technique [0002] Human serum amyloid A (SAA) is a positive reaction protein of the acute phase reaction protein species, with a molecular weight of 12,000. Its biological functions are lipid metabolism, inflammatory defense and immune response. The content of SAA in the serum of normal people is measured. When the body is infected by bacteria or viruses, it is secreted by the liver under the stimulation of interleukin 6, interleukin 1 and tumor necrosis factor. The content of SAA in the blood can be used to determine whether the infection occurs and its severity. [0003] In order to ensure the accuracy of SAA test results, reliable supporting calibrator must be available. The range of calibrator should cover 0.0-500mg / l. There is a method of isolating SAA p...

AI Technical Summary

Problems solved by technology

[0003] In order to ensure the accuracy of SAA test results, reliable supporting calibrator must be available. The range of calibrator should cover 0.0-500mg / l. There is a method of isolating SAA protein from human serum in technology, but this method is expensive and not suitable for industrial production and application. Most manufacturers choose to purchase SAA recombinant protein for the configuration of calibrator, but in the configuration process, On the one hand, there is a very significant difference with the serum matrix, which causes a large deviation in the accuracy of the measurement; on the other hand, due to the degradation of the recombinant protein and the influence of the stability of the calibrator, the measured value fluctuates greatly, which cannot meet the requirements of clinical accurate measurement

Protective agents on the market are also difficult to meet the demand

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