Electrode-replaceable in-vivo detection device for hormones in plant leaves and method of device

Abstract

The invention discloses an electrode-replaceable in-vivo detection device for hormones in plant leaves and a method of the device. The device comprises an adjustable support frame, a connecting piece, an in-vivo sampling mechanism and an electrochemical signal detector; the adjustable support frame is connected with the in-vivo sampling mechanism through the connecting piece and is matched with the connecting piece to adjust the height of the in-vivo sampling mechanism; and the electrochemical signal detector is connected with the in-vivo sampling mechanism. According to the electrode-replaceable in-vivo detection device for the hormones in plant leaves and the method of the device of the invention, an electrochemical sensing method with a three-electrode structure is adopted, and in-vivo detection is realized on the plant leaves in a non-insertion manner; besides, the in-vivo sampling mechanism is used for sampling, so that electrodes can be quickly replaced according to the types of hormones needing to be detected, and the detection of different specific plant hormones can be realized; and finally, the design that the electrochemical signal detector and the electrodes can be separated is adopted, and the working electrodes can be independently processed and secondarily modified, so that the electrochemical detection performance is improved, and the production cost and the use cost are reduced.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection, in particular to a living body detection device and method for hormones in plant leaves with replaceable electrodes. Background technique [0002] Phytohormones are trace small molecular organic substances synthesized by plants themselves. As signal molecules, they can significantly regulate plant growth and development at very low concentrations. The known plant hormones mainly include auxin, abscisic acid, gibberellin, cytokinin, ethylene, brassinolide, jasmonin, salicylic acid and strigolactone and other types. Phytohormones regulate the various processes of plant growth and development and environmental adaptation. They both independently and cooperatively regulate the growth and development processes of plant seed germination, vegetative growth, reproductive growth, embryonic development, seed maturation and dormancy, as well as the effects of biological and abiotic factors...

AI Technical Summary

Problems solved by technology

[0004] However, in the research on the detection of hormones in plant leaves by electrochemical sensors, it is usually necessary to pick the leaves to be tested for detection, and it is impossible to perform in vivo detection.

For the detection of different types of plant hormones, different working electrodes are required. The existing packaged sensors cannot replace the electrodes, and the cost of use is high.

Most of the electrochemical sensors based on screen-printed electrodes are disposable. After one use, their detection performance will be greatly reduced and cannot be reused. Moreover, the detection area of ​​the working electrode is prepared in batches using printing paste, which cannot be modified twice. In order to improve the electrochemical detection performance, it is also impossible to replace the electrodes to achieve the detection of different specific plant hormones

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