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A method for detecting fipronil residues in livestock and poultry hair

A fipronil and residual technology, which is applied in the field of detection of fipronil residual in livestock and poultry hair, achieves the effect of less sampling, simple pretreatment method and high sensitivity

Active Publication Date: 2021-11-12
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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Problems solved by technology

Therefore, research on the detection method of fipronil in livestock and poultry hair is of great significance to effectively supervise the illegal use of this type of drug, ensure the quality and safety of livestock and poultry products, and the health of consumers. Method for the detection of fipronil residues in livestock and poultry hair

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  • A method for detecting fipronil residues in livestock and poultry hair
  • A method for detecting fipronil residues in livestock and poultry hair
  • A method for detecting fipronil residues in livestock and poultry hair

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Embodiment 1

[0044]Instruments and equipment: 1290-6495 ultra-high performance liquid chromatography-electrospray tandem mass spectrometer (Agilent Corporation, USA); Milli-Q ultrapure water device (Millipore Corporation); N-EVAP112 nitrogen blowing instrument (Organomation Corporation, USA); MS205DU electronic analysis Balance (Mettler Toledo, Switzerland); GL-88B Vortex Mixer (Qilin Bell Instrument Manufacturing Company, Haimen City).

[0045] Reagent: fipronil standard: purity>98%, methanol: chromatographically pure; other reagents are analytically pure.

[0046] 1. Preparation of standard solution

[0047] Standard stock solution: Accurately weigh an appropriate amount of fipronil standard substance into a 10mL volumetric flask, dilute to volume with acetonitrile, prepare a 1.0mg / mL standard stock solution, and store at -20°C in the dark.

[0048] Standard intermediate solution: Take 1 mL of the above standard stock solution in a 10 mL volumetric flask, dilute with acetonitrile to obt...

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Abstract

The invention discloses a method for detecting the residual amount of fipronil in the hair of livestock and poultry, which uses ultra-high performance liquid chromatography-electrospray tandem mass spectrometry to detect the residual amount of fipronil in the hair of livestock and poultry, specifically using an acetonitrile solution containing 2% formic acid and water The residual fipronil in the hair is extracted. After vortexing, ultrasonication and centrifugation, the extract is dispersed and purified by the matrix of ethylenediamine-N-propylsilane and octadecylsilane, and detected by ultra-high performance liquid chromatography tandem mass spectrometry. Among them, the mobile phase A is an aqueous solution containing 5 mmol / L ammonium acetate, the mobile phase B is a methanol solution containing 5 mmol / L ammonium acetate, and the flow rate is 0.3 mL / min. The invention overcomes the disadvantages of difficult sampling and the need for live slaughter, and has strong specificity, high sensitivity, short time consumption, low requirements for sample purification, and provides reliable technical support for quality and safety supervision of livestock and poultry products.

Description

technical field [0001] The invention relates to a method for detecting fipronil residues in livestock and poultry hair, and specifically belongs to the technical field of pesticide residue detection. Background technique [0002] Fipronil is a broad-spectrum phenylpyrazole insecticide. It is mainly stomach-toxic to pests. Its mechanism of action is to hinder the chloride metabolism controlled by insect γ-aminobutyric acid, so it has a high insecticidal activity. Animal experiments have shown that eating animal foods containing high concentrations of fipronil residues (such as meat, eggs, milk, vegetables, etc.) can cause damage to the nervous system, liver, thyroid, and kidneys, and has carcinogenic, teratogenic, and mutagenic effects. role. EU law stipulates that fipronil shall not be used in the livestock and poultry breeding process of the human food industry chain. Announcement No. 1157 of the Ministry of Agriculture of my country stipulates that the sale and use of p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 徐俊闵佳玲周瑶敏胡丽芳谢敏肖勇陈庆隆
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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