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High-disease-resistance tissue culture seedling raising method for polygonatum sibiricum

A technology of disease resistance and Polygonatum, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of susceptibility to infection, poor disease resistance of seedlings, loss, etc.

Inactive Publication Date: 2021-05-18
云南帅豪农牧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] What's more serious is that in the process of artificial breeding, the seedlings have poor disease resistance and are easily infected, causing losses.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]1) Take materials, take one of the rhizomes of Polygonatum and the shoots on the ground for activation treatment, cut into sections after processing, and obtain the section of Polygonatum at 0.5-1 cm;

[0042] 2) Induction, disinfect the Polygonatum segment, move 1 to 3 Polygonatum segments into the basic culture medium, spray the bacterial liquid, spray twice with an interval of 15 hours, spray 0.5-1ml for the first time, and 1-2ml for the second time, Cultivation in the culture room, the light cycle starts and ends from 8:00 am to 8:00 pm, the daily temperature is 20-22°C, the night temperature is 17-18°C, the light intensity is 2000-2400lux, the humidity is 55-58%, and the culture is 12-15 days;

[0043] 3) Subculture, inoculate the induced tissue with good growth state into the proliferation medium and culture it in the dark for 5-8 days at a temperature of 20-23°C, and culture it under the condition of 3000 lux of light for 6-8 hours, Then adjust the light to 2500lu...

Embodiment 2

[0055] 1) Take materials, take one of the rhizomes of Polygonatum and the shoots on the ground for activation treatment, cut into sections after processing, and obtain the section of Polygonatum at 0.5-1 cm;

[0056] 2) Induction, disinfect the Polygonatum segment, move 1 to 3 Polygonatum segments into the basic culture medium, spray the bacterial liquid, spray twice with an interval of 15 hours, spray 0.5-1ml for the first time, and 1-2ml for the second time, Cultivation in the culture room, the light cycle starts and ends from 8:00 am to 8:00 pm, the daily temperature is 20-22°C, the night temperature is 17-18°C, the light intensity is 2000-2400lux, the humidity is 55-58%, and the culture is 12-15 days;

[0057] 3) Subculture, inoculate the induced tissue with good growth state into the proliferation medium and culture it in the dark for 5-8 days at a temperature of 20-23°C, and culture it under the condition of 3000 lux of light for 6-8 hours, Then adjust the light to 2500l...

Embodiment 3

[0069] 1) Take materials, take one of the rhizomes of Polygonatum and the shoots on the ground for activation treatment, cut into sections after processing, and obtain the section of Polygonatum at 0.5-1 cm;

[0070] 2) Induction, disinfect the Polygonatum segment, move 1 to 3 Polygonatum segments into the basic culture medium, spray the bacterial liquid, spray twice with an interval of 15 hours, spray 0.5-1ml for the first time, and 1-2ml for the second time, Cultivation in the culture room, the light cycle starts and ends from 8:00 am to 8:00 pm, the daily temperature is 20-22°C, the night temperature is 17-18°C, the light intensity is 2000-2400lux, the humidity is 55-58%, and the culture is 12-15 days;

[0071] 3) Subculture, inoculate the induced tissue with good growth state into the proliferation medium and culture it in the dark for 5-8 days at a temperature of 20-23°C, and culture it under the condition of 3000 lux of light for 6-8 hours, Then adjust the light to 2500l...

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Abstract

The invention discloses a high-disease-resistance tissue culture seedling raising method for polygonatum sibiricum. The high-disease-resistance tissue culture seedling raising method for polygonatum sibiricum comprises the following steps: 1) material taking, 2) induction, 3) subculture, 4) differentiation culture, 5) seedling strengthening and rooting culture and 6) transplanting: selecting seedling plants which grow robustly and are good in rooting, exercising seedlings for 2-3 days, and transplanting the seedlings into a planting nursery. The high-disease-resistance tissue culture seedling raising method improves disease resistance of polygonatum sibiricum, can effectively resist black spot and leaf spot of the polygonatum sibiricum, enhances a disease-resistant advantage of the polygonatum sibiricum, and effectively increases a survival rate of the planted polygonatum sibiricum.

Description

technical field [0001] The invention relates to the technical field of planting, in particular to a method for cultivating seedlings of Polygonatum with high disease resistance. Background technique [0002] Polygonatum is a herbaceous plant of the genus Polygonatum in the family Liliaceae, and it is one of the three basic plants of Polygonatum commonly used in traditional Chinese medicine. Polygonatum, as a commonly used traditional Chinese medicine, has a history of more than 2,000 years in my country. Its nature and taste are sweet and flat, and it enters the lung, spleen and kidney meridians. Efficacy Buzhongyiqi, moisten the heart and lungs, strengthen the bones and muscles. Control cold and heat due to deficiency, tuberculosis and hemoptysis, lack of food after illness, weakness of muscles and bones, rheumatism pain, wind leprosy and ringworm disease, it is a product for nourishing yin. Pharmacological studies in recent years have shown that, in addition to the above...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 张远东李建华
Owner 云南帅豪农牧科技有限公司
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