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Clostridium butyricum liquid fermentation preparation method and liquid culture medium

A fermentation medium, the technology of Clostridium butyricum, applied in the field of liquid fermentation culture and fermentation technology of anaerobic microorganisms

Active Publication Date: 2021-05-14
XIAMEN HUIYING ANIMAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to overcome the shortcomings of the existing Clostridium butyricum fermentation technology, the present invention provides a preparation method for Clostridium butyricum liquid fermentation, aiming to solve how to quickly create an anaerobic environment and optimize the production process to improve the production rate of Clostridium butyricum The number of live bacteria

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  • Clostridium butyricum liquid fermentation preparation method and liquid culture medium

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Effect test

Embodiment 1

[0027] Clostridium butyricum was taken out from the -80°C refrigerator and inoculated into 3L seed medium, and cultured at 37°C for 18 hours. Afterwards, insert 2% of the inoculum into the 150L fermentation medium of the 200L fermenter for anaerobic culture at 37°C, turn on the motor for stirring, the speed is 80r / min, and open the valve of the air liquid seal device, and the culture time is 20h, and the activity of Clostridium butyricum is detected. The number of bacteria and the pH value of the fermentation broth.

[0028] Wherein, the seed medium is: glucose monohydrate 5g / L, soluble starch 10g / L, yeast extract 10g / L, dipotassium hydrogen phosphate 2g / L, calcium carbonate 8g / L, tomato powder 0.5g / L, pH7.0. The fermentation medium is: glucose monohydrate 5g / L, soluble starch 10g / L, yeast extract 10g / L, dipotassium hydrogen phosphate 2g / L, calcium carbonate 8g / L, tomato powder 0.5g / L, pH7. 0. Set different concentration gradients for ferrous sulfate: 0g / L, 2g / L, 4g / L, 6g / L...

Embodiment 2

[0034] Clostridium butyricum was taken out from the -80°C refrigerator and inoculated into 3L seed medium, and cultured at 37°C for 18 hours. Afterwards, insert 2% inoculum amount into 150L fermentation medium in a 200L fermenter for anaerobic culture at 37°C, turn on the motor for stirring, the rotation speed is 80r / min, and the culture time is 20h. The ingredients of the seed medium and fermentation medium are: glucose monohydrate 5g / L, soluble starch 10g / L, yeast extract 10g / L, dipotassium hydrogen phosphate 2g / L, tomato powder 0.5g / L, pH7.0 . Ferrous sulfate and calcium carbonate are not added in the culture medium of this embodiment, and the air liquid seal device is not used in the fermentation process.

Embodiment 3

[0036]Clostridium butyricum was taken out from the -80°C refrigerator and inoculated into 3L seed medium, and cultured at 37°C for 18 hours. Afterwards, insert 2% of the inoculum into 150L fermentation medium in a 200L fermenter for anaerobic culture at 37°C, turn on the motor for stirring at a speed of 80r / min, and open the valve of the air liquid seal device for 20h of culture time. The composition of the seed medium and fermentation medium is: glucose monohydrate 5g / L, soluble starch 10g / L, yeast extract 10g / L, dipotassium hydrogen phosphate 2g / L, ferrous sulfate 8g / L, calcium carbonate 8g / L, tomato powder 0.5g / L, pH7.0. This embodiment is the solution of the present invention.

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Abstract

The invention discloses a clostridium butyricum liquid fermentation preparation method which comprises the following steps: taking clostridium butyricum as a fermentation strain, inoculating the strain taken out from a refrigerator at the temperature of -80 DEG C into a 3L seed culture medium, and standing and culturing at the temperature of 37 DEG C for 18 hours; inoculating the seed solution into 150L of fermentation culture medium of a 200L fermentation tank according to the inoculum size of 2%, performing anaerobic culture at 37 DEG C, starting a motor for stirring, and opening a valve of an air liquid sealing device; the seed culture medium and the fermentation culture medium comprising the following components: 3-5g / L of dextrose monohydrate, 5-10g / L of soluble starch, 5-10g / L of yeast extract, 1-3g / L of dipotassium phosphate, 6-10g / L of ferrous sulfate, 5-10g / L of calcium carbonate and 0.2-0.8 g / L of tomato powder, and the pH value is 6.5-7.0. Ferrous sulfate, calcium carbonate and an air liquid sealing device supplement one another and have a synergistic effect, so that the interior of a fermentation tank and a fermentation culture medium rapidly reach an anaerobic environment, the inhibition effect of oxygen on the growth of clostridium butyricum is relieved, the clostridium butyricum is promoted to rapidly grow and propagate, the viable count of the clostridium butyricum can be increased from 7.6 * 10<8> CFU / mL to 4.1 * 10<9> CFU / mL, and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of liquid fermentation culture and fermentation technology of anaerobic microorganisms, in particular to a preparation method of Clostridium butyricum liquid fermentation. Background technique [0002] Clostridium butyricum, also known as butyric acid bacteria, belongs to the genus Clostridium and is an obligate anaerobic Gram-positive bacillus. It can ferment glucose, sucrose, lactose and other carbohydrates to produce acid, and can produce amylase, and then hydrolyze starch to produce butyric acid, acetic acid and lactic acid. Clostridium butyricum can not only promote the growth and metabolism of probiotics such as Bifidobacterium and Lactobacillus in the intestinal tract of animals, but also inhibit the growth and reproduction of harmful bacteria and spoilage bacteria in the intestinal tract, so as to improve intestinal health and maintain Balance the probiotic flora and reduce the accumulation of intes...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/145
CPCC12N1/20
Inventor 林松泉庄若飞庄建军邹文政曾海燕刘兵
Owner XIAMEN HUIYING ANIMAL TECH
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