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Peony petal extract as well as preparation method and application thereof

A technology of peony petals and extracts, applied in the biological field, can solve problems such as the lack of analysis of the medicinal value of peony petals extracts

Active Publication Date: 2021-05-14
瑞莱茵(北京)生物科技有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The peony petal products or preparations disclosed above are all based on the traditional understanding and efficacy of peony petals, the preparation method is relatively simple, and there is no analysis of the medicinal value of peony petal extracts

Method used

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  • Peony petal extract as well as preparation method and application thereof
  • Peony petal extract as well as preparation method and application thereof
  • Peony petal extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, prepare peony petal extract A

[0036] One, the preparation method of peony petal extract A

[0037] The preparation method of peony petal extract A comprises the following steps:

[0038] (1) Dry and pulverize peony petals, add malic acid-ethanol-water solution with a volume ratio of 3:67:30 according to the ratio of material to liquid 1g:15ml, ultrasonically extract twice at a temperature of 20°C, 30min each time, collect and combine extract to obtain crude extract;

[0039] (2) The crude extract obtained in step (1) is concentrated under reduced pressure at a temperature of 50° C. and a pressure of 0.05 MPa until there is no alcohol smell, diluted with water to a relative density of 1.10, and added with a concentration of 1% by volume. Aqueous sodium hydroxide solution, adjust the pH value of the solution to 13.0, filter to remove insoluble matter, extract the filtered solution with ethyl acetate for 3 times, and remove the ethyl acetate extract to ob...

Embodiment 2

[0052] Embodiment 2, prepare peony petal extract B

[0053] One, the preparation method of peony petal extract B

[0054] The preparation method of peony petal extract B comprises the following steps:

[0055] (1) Dry and pulverize the peony petals, add acetic acid-ethanol-water solution with a volume ratio of 5:80:15 according to the ratio of material to liquid 1g:20ml, and extract ultrasonically for 3 times at a temperature of 25°C, each time for 30min and 20min respectively , 10min, collect and combine extracts, obtain crude extracts;

[0056] (2) The crude extract obtained in step (1) is concentrated under reduced pressure at a temperature of 30°C and a pressure of 0.1 MPa until it has no alcohol smell, diluted with water to a relative density of 1.20, and added with a concentration of 3% by volume. An aqueous solution of potassium hydroxide, adjusting the pH value of the solution to 11.0, filtering to remove insoluble matter, extracting the filtered solution with ethyl ...

Embodiment 3

[0065] Embodiment 3, prepare peony petal extract C

[0066] One, the preparation method of peony petal extract C

[0067] The preparation method of peony petal extract C comprises the following steps:

[0068] (1) Dry and pulverize peony petals, add formic acid-ethanol-water solution with a volume ratio of 4:76:20 according to the ratio of material to liquid 1g:12ml, ultrasonically extract 3 times at a temperature of 30°C, each time for 15min, collect and extract liquid to obtain the crude extract;

[0069](2) The crude extract obtained in step (1) is concentrated under reduced pressure at a temperature of 60°C and a pressure of 0.01 MPa until it has no alcohol smell, diluted with water to a relative density of 1.10, and added with a volume percentage concentration of 2% Potassium hydroxide aqueous solution, adjust the pH value of the solution to 12.0, filter to remove insoluble matter, extract the filtered solution with ethyl acetate for 3 times, and remove the ethyl acetat...

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Abstract

The invention discloses a peony petal extract as well as a preparation method and the application thereof. The preparation method of the peony petal extract provided by the invention comprises the following steps: (1) drying and crushing peony petals, adding an organic acid-organic solvent-water mixed solvent for ultrasonic extraction, and collecting and combining extracting solutions to obtain a crude extracting solution; (2) concentrating the crude extract obtained in the step (1) under reduced pressure until no organic solvent smell exists, adding an aqueous alkali solution, adjusting the pH value of the solution to 11.0-13.0, filtering to remove insoluble substances, extracting the filtered solution with ethyl acetate, and removing the ethyl acetate extract to obtain an aqueous alkali extract; and (3) acidifying the alkaline water extract obtained in the step (2) with acid water, extracting with ethyl acetate to obtain an ethyl acetate extract, removing the solvent, and drying to obtain the target extract.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to peony petal extract and its preparation method and application. Background technique [0002] Peony petals can be soaked in water for drinking, which has certain pharmacological effects. The theory of traditional Chinese medicine believes that drinking some peony petal tea in an appropriate amount can regulate the health of the body, and it is a scented tea drink with good efficacy. CN103494206B discloses a preparation process for soft capsules containing peony petal isoflavones and peony seed essential oil. In the provided preparation process, peony petal isoflavones are extracted by biological methods. CN108452035A discloses a method for extracting polyphenols from peony petals, which includes the steps of raw material pretreatment, polyphenol extraction and crude liquid centrifugation, drying peony petals, making powder, and sieving, taking peony petal fine powder and volume frac...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K36/65A61K8/9789A61P35/00A61Q19/00A23L33/105A61K133/00
CPCA61K36/71A61K36/65A61K8/9789A61P35/00A61Q19/00A23L33/105A61K2236/333A61K2800/82A23V2002/00A23V2300/14A23V2300/48A23V2250/21A23V2200/308
Inventor 舒庆艳马晓丰方令豪
Owner 瑞莱茵(北京)生物科技有限责任公司
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