Application of dihydrocoumarin to inhibition of seed germination and growth of barnyard grass
A technology of dihydrocoumarin and barnyardgrass, which is applied in the directions of application, herbicides and algicides, plant growth regulators, etc., can solve the problems such as the literature and data on the prevention and control of barnyardgrass has not yet been found.
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Embodiment 1
[0023] Example 1 Petri dish detection method to measure the influence of dihydrocoumarin on the germination and growth of barnyardgrass seeds
[0024] (1) Breaking dormancy and disinfection of seeds: Treat the collected barnyardgrass seeds with concentrated sulfuric acid for 4-5 minutes, after cleaning, soak them in 3% sodium hypochlorite and 75% ethanol for 1-2 minutes. After the sterilization is completed, rinse with clean water, filter paper to dry the surface moisture, and set aside.
[0025] The germination rate of treated barnyardgrass seeds is higher than 85%.
[0026] (2) Solution preparation: dissolve dihydrocoumarin with a small amount of absolute ethanol, and prepare dihydrocoumarin solutions with concentrations of 1000, 500, 100, 50, 10 and 0 mg / l with pure water.
[0027] (3) Petri dish detection method: pad 2 layers of filter paper in a 9cm disposable petri dish, add 5ml of dihydrocoumarin solutions of different concentrations, put 10 full-grained barnyardgrass ...
Embodiment 2
[0036] Example 2 Pot detection method to determine the effect of dihydrocoumarin on the germination and growth of barnyardgrass seeds
[0037] (1) Breaking dormancy and disinfection of seeds: Treat the collected barnyardgrass seeds with concentrated sulfuric acid for 4-5 minutes, after cleaning, soak them in 3% sodium hypochlorite and 75% ethanol for 1-2 minutes. After the sterilization is completed, rinse with clean water, filter paper to dry the surface moisture, and set aside.
[0038] The germination rate of treated barnyardgrass seeds is higher than 85%.
[0039] (2) Solution preparation: dissolve dihydrocoumarin with a small amount of absolute ethanol, and prepare dihydrocoumarin solutions with concentrations of 2000, 1000, 500, 100, 50 and 0 mg / l with pure water.
[0040] (3) Put 1 / 2 cup of air-dried and sieved field soil in a plastic round basin with a diameter of 8.6 cm and a height of 6 cm, and add enough water to completely soak the field soil for more than 2 hours...
Embodiment 3
[0051] A product for inhibiting germination and growth of barnyardgrass seeds, which includes not only products using dihydrocoumarin alone but also products using dihydrocoumarin as an active ingredient. The product may also include acceptable carriers and auxiliary materials, which include one or more of antioxidants, chelating agents, surfactants, fillers, disintegrants, wetting agents, solvents and pH regulators. The dosage form of the product can be powder, granule, wettable powder, soluble powder, water dispersible granule, water suspension, emulsifiable concentrate, water emulsion, microemulsion, granule, water or tablet. The selection of the above-mentioned carriers, auxiliary materials and dosage forms are all conventional technical means in the art.
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