Kit and method for joint detection based on microspheres with different fluorescence intensities

A fluorescence intensity and combined detection technology, which is applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve problems such as inability to detect, and achieve full response, accurate results, and stable detection results.

Pending Publication Date: 2021-05-07
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the above-mentioned methods are all aimed at the detection of a single pathogen index, and the disadvantage is that large-scale detection equipment is required, and in particular, it is not possible to detect multiple pathogen indicators on one specimen in a short period of time.

Method used

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  • Kit and method for joint detection based on microspheres with different fluorescence intensities
  • Kit and method for joint detection based on microspheres with different fluorescence intensities
  • Kit and method for joint detection based on microspheres with different fluorescence intensities

Examples

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Effect test

Embodiment 1

[0030] A combined detection kit based on microspheres with different fluorescence intensities, the kit includes microsphere reagents, labeled second antibodies or antigens; the microsphere reagents are N types of microspheres with the same diameter and different fluorescence intensities, The surface of the sphere corresponds to the first antibody or antigen coated with N blood markers, where N is an integer between 2 and 5; the labeled second antibody or antigen includes the second antibody or antigen of N blood markers, and the second The antibody or antigen is labeled with fluorescein, and the binding sites of the first antibody or antigen, the second antibody or antigen and the corresponding antigen or antibody are different.

[0031] When N is 5, the preparation method of this microsphere reagent comprises the steps:

[0032] Step 101: Select five microspheres with the same diameter and different fluorescence intensity codes, and coat five different specific antigens or an...

Embodiment 2

[0093] A joint detection method based on microspheres of different fluorescence intensities, the method utilizes the kit for joint detection based on microspheres of different fluorescence intensities in Example 1, specifically comprising the following steps, such as Figure 1-2 Shown:

[0094] S101: adding the sample to be tested into the kit and incubating with the microsphere reagent inside to form a first reactant; the sample to be tested is one of whole blood and serum.

[0095] It should be understood that the microsphere reagent is the microsphere reagent mentioned in Example 1.

[0096] S102: adding a labeled second antibody or antigen to the first reactant for incubation to obtain a second reactant;

[0097] It should be understood that the labeled secondary antibody or antigen is prepared in the manner mentioned in Example 1.

[0098] S103: Detect the fluorescent signals of the blood markers on the surface of the N immune microspheres in the second reactant, so as ...

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Abstract

The invention provides a kit for joint detection based on microspheres with different fluorescence intensities. The kit comprises a microsphere reagent, and a labeled second antibody or antigen; the microsphere reagent is N microspheres with the same diameter and different fluorescence intensity codes, the surfaces of the N microspheres are correspondingly coated with first antibodies or antigens of N blood markers, and N is an integer between 2 and 5; and the labeled second antibody or antigen comprises N second antibodies or antigens of blood markers, fluorescein is labeled on the second antibodies or antigens, and the binding sites of the first antibody or antigen and the corresponding antigens or antibodies and the binding sites of the second antibodies or antigens and the corresponding antigens or antibodies are different. The invention also relates to a method for joint detection based on microspheres with different fluorescence intensities. The kit provided by the invention can realize specific detection of five pathogen antigens / antibodies in blood transfusion through one-time reaction, and the detection process is simple, convenient and accurate.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a combined detection kit and method based on microspheres with different fluorescence intensities. Background technique [0002] Blood safety is an important field related to people's life and health. Clinical transfusion-transmitted infection of hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), Treponema pallidum (TP), human T cell Virus (HTLV) and other diseases have attracted great attention from the state and society. To clarify the infection status of patients with hepatitis B, hepatitis C, AIDS, and syphilis before operation and blood transfusion can provide timely diagnosis basis for the clinic, avoid blood-borne infection caused by operation and blood transfusion during operation, and improve the medical staff's health during treatment. Self-protection awareness helps to reduce occupational exposure, reduce the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/571G01N33/543G01N33/535G01N21/64
CPCG01N33/56983G01N33/56988G01N33/571G01N33/54313G01N33/54306G01N33/535G01N21/6428G01N2021/6439G01N2333/15G01N2333/02G01N2333/186
Inventor 段生宝李勇蒙青林王红梅陈晔洲丁少华魏双施王玉珏田晶晶刘杰王泽龙
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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