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Composite porous microspheres and preparation method and application thereof

A technology of porous microspheres and chitosan, which is applied in the field of medicine, can solve problems such as microspheres that cannot be prepared for vascular embolism, and achieve the effect of being suitable for large-scale preparation, easy to repeat, and uniform in shape and size

Pending Publication Date: 2021-05-07
森心(上海)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main size distribution of the prepared microspheres is at the nanoscale, which cannot be more than 2 μm, and it is impossible to prepare microspheres for vascular embolism at tumor sites. This document does not involve the problem of water absorption and swelling of microspheres.

Method used

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  • Composite porous microspheres and preparation method and application thereof
  • Composite porous microspheres and preparation method and application thereof
  • Composite porous microspheres and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] (1) Dissolve 1g chitosan oligosaccharide in 25mL DMF, add 5mL chlorosulfonic acid, adjust the pH to 7.0 after reacting at 70°C for 4h, wash with deionized water and ethanol three times respectively, centrifuge and dialyze with 2500Da dialysis bag for 48h, then vacuum Freeze-drying to obtain sulfonated chitosan;

[0088] (2) Weigh 0.625g chitosan, 0.1875g sulfonated chitosan and 0.1875g sodium alginate respectively, and prepare a mixed aqueous solution with a mass fraction of 1%;

[0089] (3) Contain 4.8g Span 80 and 0.2g Tween 20 in 100mL petroleum ether for preparing emulsifier solution;

[0090] (4) Weigh 0.08gCaCl 2 , prepared into a 5mL aqueous solution. Add 10mL chitosan / sulfonated chitooligosaccharide / sodium alginate aqueous solution drop by drop into the organic solvent containing emulsifier through a syringe pump, stir evenly to form an emulsion, the rotation speed is 520rpm; after stirring at a constant speed for 3h, add the prepared CaCl 2 Aqueous solution,...

Embodiment 2

[0095] (1) Take by weighing 0.5g chitosan, 0.25g sulfonated chitosan and 0.25g sodium alginate respectively, and prepare a mixed aqueous solution with a mass fraction of 1%;

[0096] (2) Contain 4.5g Span 80 and 0.5g Tween 20 in 100mL petroleum ether for preparing emulsifier solution;

[0097] (3) Add 10mL chitosan / sulfonated chitooligosaccharide / sodium alginate aqueous solution dropwise into the organic solvent containing emulsifier through a syringe pump, and stir evenly to form an emulsion at a speed of 520rpm;

[0098] (4) After stirring at a constant speed for 3 hours, add 5 mL of 0.08 g / mL CaCl 2 Aqueous solution, 100 μL 50% (v / v) glutaraldehyde, stirred for 3 hours, added 100 μL 10% (v / v) terephthalaldehyde, continued to stir for 6 hours;

[0099] (5) Thermally induce phase separation of the above emulsion at -80°C-20°C, freeze treatment for 4 hours, then remove the upper layer of the emulsion, leaving the lower layer of microspheres, and then wash with deionized water...

Embodiment 3

[0103] (1) Take by weighing 0.142g chitosan, 0.429g sulfonated chitosan and 0.429g sodium alginate respectively, and prepare a mixed aqueous solution whose mass fraction is 1%;

[0104] (2) Prepare an emulsifier solution containing 4.8g Span 80 and 0.2g Tween 20 in 100mL petroleum ether;

[0105] (3) Add 10mL chitosan / sulfonated chitooligosaccharide / sodium alginate aqueous solution dropwise into the organic solvent containing emulsifier through a syringe pump, and stir evenly to form an emulsion at a speed of 520rpm;

[0106] (4) After stirring at a constant speed for 3 hours, add 7.5mL of 0.08g / mL CaCl 2 Aqueous solution, 50 μL of 50% (v / v) glutaraldehyde, stirred for 3 hours, added 100 μL of 10% (v / v) terephthalaldehyde, and continued to stir for 12 hours;

[0107] (5) Thermally induce phase separation of the above emulsion at -80°C-20°C, freeze treatment for 4 hours, then remove the upper layer of the emulsion, leaving the lower layer of microspheres, and then wash with de...

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Abstract

The invention relates to composite porous microspheres and a preparation method and application thereof. According to a composite porous microsphere preparation and a preparation method and application thereof, the composite porous microsphere preparation comprises the following components: chitosan and derivatives thereof, sulfonated chitosan oligosaccharide and sodium alginate. The prepared microspheres of the invention have the advantages of being easy to collect, adjustable in size and the like, and not only have a good swelling degree suitable for embolism, but also have high drug loading capacity; release of the drug loaded on the microspheres at a tumor part can be used for tumor treatment, so that the physical therapy effect on the tumor is further enhanced.

Description

technical field [0001] The invention relates to a composite porous microsphere, in particular to a composite porous microsphere based on chitosan / sulfonated chitooligosaccharide / sodium alginate and its use as an embolism, which belongs to the field of medicine. Background technique [0002] In the current market, chitosan, also known as deacetylated chitin, is the product of deacetylation of chitin and is one of the most abundant natural polymers on the earth. This high polymer has good biocompatibility and can be Degradability, bioadhesion, anti-oxidation, anti-allergic, anti-inflammatory, antibacterial, hemostasis, promotion of wound healing, reduction of scar hyperplasia and other biological functions have attracted wide attention in various industries. , food, biomedical engineering and many other fields have a wide range of applications. [0003] Sodium alginate is a natural polysaccharide, which is a by-product after extracting iodine and mannitol from brown algae kel...

Claims

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Application Information

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IPC IPC(8): A61L24/08A61L24/00
CPCA61L24/043A61L24/08A61L24/0036A61L24/001A61L24/0015A61L2300/416A61L2430/36C08L5/08C08L5/04
Inventor 段友容张伟武志华赵德王琪
Owner 森心(上海)科技有限公司
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