Ovulation detection test strip and detection method

Abstract

The invention discloses an ovulation detection test strip. The ovulation detection test strip comprises a PVC bottom plate, a sample pad, a gold label pad, a nitrocellulose film and a water absorption pad, wherein the nitrocellulose film is provided with a detection line T1, a detection line T2 and a quality control line, the detection line T1 is coated with a mouse anti-human beta LH monoclonal antibody 1A4, the detection line T2 is coated with a cotinine antigen, and the quality control line is coated with a goat anti-chicken IgY polyclonal antibody; and a colloidal gold mouse anti-human beta LH monoclonal antibody 6B8 conjugate, a colloidal gold chicken IgY antibody conjugate and a colloidal gold cotinine monoclonal antibody conjugate are cured on the gold label pad. The test strip is high in detection sensitivity and good in specificity, abnormal interpretation results caused by crossing of LH with follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH) can be avoided, detection results are more accurate, and monitoring of the smoking metabolite cotinine in urine is combined. The invention further discloses an ovulation detection method, the steps are simple, and the test result is accurate.

Description

technical field [0001] The invention relates to an ovulation detection test paper, in particular to an ovulation detection test paper strip and a detection method. Background technique [0002] Luteinizing hormone (LH) is a substance secreted by basophils of the pituitary gland. In females (LH) works in concert with follicle-stimulating hormone (FSH) to maintain the ovarian menstrual cycle, leading to ovulation and corpus luteum formation. The production of LH is controlled by the hypothalamic gonadotropin-releasing hormone, and is also regulated by positive and negative feedback from the ovary. The release peak of LH in the menstrual cycle is closely related to ovarian ovulation. Once the LH peak appears, it indicates ovarian ovulation in 24-36 hours. Therefore, the peak serum LH can be monitored during the menstrual cycle to determine the best time for conception. The test results are expressed in milliinternational units / milliliter (mIu / ml). [0003] LH is a glycoprote...

AI Technical Summary

Problems solved by technology

[0004] The current method for detecting luteinizing hormone (LH) is time-resolved immunofluorescence (patent application number: 200810043552.5). Non-specific fluorescence, but this method requires the use of rare earth metals, which is costly and difficult to label

[0005] The patent application number 201210571305.9 uses the method of labeling fluorescein to detect luteinizing hormone. The kit has a short validity period and poor stability

[0006] Chinese invention patent CN201310237681.9 discloses a Luteinizing Hormone Nano-Magnetic Particle Chemiluminescent Immunoquantitative Detection Kit. The kit is complicated to use and requires a chemiluminescence instrument, which is not suitable for home monitoring.

[0007] The colloidal gold method for detecting ovulation test strips has become the mainstream of home self-examination. It is easy to operate and interpret, and does not require sample dilution and equipment. The base structure is the same, it is easy to cause crossover and lead to abnormal interpretation of results

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