Method for detecting sulfur stable isotope of inflorescence axis tissue culture garlic

A stable isotope, tissue culture technology, used in measurement devices, material analysis by electromagnetic means, instruments, etc., can solve problems such as difficult to focus receivers and affect detection results, achieve good reproducibility, improve accuracy and reproducibility. Realistic and accurate results

Inactive Publication Date: 2021-04-30
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When switching from carbon and nitrogen isotope detection to sulfur isotope detection, due to the influence of the memory effect of the instrument, SO with a high mass-to-charge ratio 2 + Difficult to effectively focus on the receiver, affecting detection results

Method used

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  • Method for detecting sulfur stable isotope of inflorescence axis tissue culture garlic
  • Method for detecting sulfur stable isotope of inflorescence axis tissue culture garlic
  • Method for detecting sulfur stable isotope of inflorescence axis tissue culture garlic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for detecting stable isotopes of garlic sulfur in inflorescence axis tissue culture, comprising the following steps:

[0023] a. After drying, grinding and filtering the garlic bulb sample of the inflorescence rachis tissue, accurately weigh 5.0 mg of the sample, put it into a tin cup and wrap it;

[0024] b. IAEA-S-1 (silver sulfide, δS34=-0.30‰), IAEA-S-3 (silver sulfide, δS34=-32.3‰), NBS-127 (barium sulfate, δS34=20.3‰) is the standard product.

[0025] c. Use an element analyzer-stable isotope mass spectrometer to detect the sample to be tested; the parameters of the element analyzer are: oxidation tube 1000 ° C, column oven 80 ° C, the catalyst in the quartz tube in the oxidation furnace is 25 grams of tungsten oxide and granular 50 grams of pure copper.

[0026] d. The high-energy ion source ionizes the sample to generate SO + The parameters of the ion source of the stable isotope mass spectrometer are: the core voltage of the ion source is 5.8KV, and...

Embodiment 2

[0034] A method for detecting stable isotopes of garlic sulfur in inflorescence axis tissue culture, comprising the following steps:

[0035] a. After drying, grinding, and filtering the garlic bulb samples of inflorescence axis tissue culture, 1.0, 2.5, 4.0, 5.5, 7.0, 8.5, and 10.0 mg samples were accurately weighed into tin cups and wrapped.

[0036] b. IAEA-S-1 (silver sulfide, δS34=-0.30‰), IAEA-S-3 (silver sulfide, δS34=-32.3‰), NBS-127 (barium sulfate, δS34=20.3‰) is the standard product.

[0037] c. Use an element analyzer-stable isotope mass spectrometer to detect the sample to be tested; the parameters of the element analyzer are: oxidation tube 1000 ° C, column oven 80 ° C, the catalyst in the quartz tube in the oxidation furnace is 25 grams of tungsten oxide and granular 50 grams of pure copper.

[0038] d. The high-energy ion source ionizes the sample to generate SO+ The parameters of the ion source of the stable isotope mass spectrometer are: the core voltage of...

Embodiment 3

[0046] comparative test

[0047]

[0048] In summary, the method of the present invention has the advantages of high precision, good repeatability, and high efficiency, and can improve the ability to detect sulfur isotopes in garlic samples cultured from inflorescence rachis tissue.

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Abstract

The invention discloses a method for detecting sulfur stable isotope of inflorescence axis tissue culture garlic in an SO+ mode, which comprises the following specific steps: drying, grinding and filtering an inflorescence axis tissue culture garlic bulb sample by using a screen, filling into a tin cup, wrapping, and detecting a sample to be detected by using an element analyzer-stable isotope mass spectrometer. According to the method, the secondary ionization effect of ion source high-energy electron flow on SO2+ is utilized, and the generated SO+ is efficiently separated and accurately detected by tuning the voltage-carrying pressure of the quadrupole mass analyzer, so that the aim of efficiently and accurately detecting the sulfur isotope ratio of the garlic subjected to inflorescence axis tissue culture is fulfilled, and the method is high in result precision, good in repeatability and high in efficiency.

Description

[0001] The invention was funded by the 2021 Agricultural Achievement Transformation and Promotion Project of the Tianjin Agricultural Commission. technical field [0002] The invention belongs to the technical field of stable isotope analysis, and in particular relates to a method for detecting garlic sulfur stable isotope. Background technique [0003] Garlic is an important spicy vegetable variety on the table of our people, and has important edible and medicinal values. A number of studies have confirmed that a variety of biologically active substances contained in garlic, such as allicin and polysaccharides, have significant anti-virus, anti-cancer cell activity, anti-thrombosis, lowering blood lipids, lowering blood pressure, and alleviating atherosclerosis. [0004] Plant tissue culture refers to the cultivation of isolated plant organs, tissues, cells or protoplasts under sterile environment conditions, artificially controlling temperature, light, humidity, nutrition,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 王振英刘晓颖范宝莉尚云涛耿以工田立鹏
Owner TIANJIN NORMAL UNIVERSITY
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