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Electrochemical immunosensor for detecting tiamulin antigen and preparation method thereof

An immunosensor, tiamulin technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problem of few electrochemical immunoassay methods, achieve faster electron transfer, improve sensitivity, and lower detection limit Effect

Inactive Publication Date: 2021-04-30
河南中泽生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the rapid detection of TIA mainly focuses on the traditional enzyme-linked immunosorbent assay and colloidal gold immunochromatography, while the research on electrochemical immunoassay is relatively few.

Method used

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  • Electrochemical immunosensor for detecting tiamulin antigen and preparation method thereof
  • Electrochemical immunosensor for detecting tiamulin antigen and preparation method thereof
  • Electrochemical immunosensor for detecting tiamulin antigen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) The gold electrode is used as the basic electrode, and Al of 1.0, 0.3 and 0.05 μm 2 o 3 Grind the polishing powder to a mirror surface, then sonicate in absolute ethanol for 3 minutes, N 2 blow dry;

[0047] (2) Dissolve 10 mg of graphene oxide in an aqueous solution and ultrasonically disperse it for 3 hours, then add 0.5 g of glucose to the above aqueous solution under stirring to obtain solution A; 0.55M NH 3 ·H 2 O was slowly added to 6.7 mL with a concentration of 0.18 MAgNO 3 solution, solution B was obtained; solution B was slowly added to solution A, magnetically stirred for 8 minutes and then left at room temperature for 1.5 hours to obtain solution C; solution C was centrifuged at 12000 rpm for 5 minutes, the supernatant was discarded, and washing the precipitate with deionized water several times until the pH of the solution is neutral, and then drying the washed precipitate at 60°C for 16 hours to obtain the silver-graphene oxide nanomaterial;

[00...

Embodiment 2

[0068] (1) With the gold electrode as the basic electrode, 1.0, 0.3 and 0.05 μm Al 2 o 3 The polishing powder is polished to a mirror surface, and then ultrasonicated in deionized water for 5 minutes, N 2 blow dry;

[0069] (2) Dissolve 10 mg of graphene oxide in an aqueous solution and ultrasonically disperse it for 1 hour, then add 0.7 g of glucose to the above aqueous solution under stirring to obtain solution A; 3 ·H 2 O was slowly added to 8 mL of 0.06M AgNO 3 solution, solution B was obtained; solution B was slowly added to solution A, magnetically stirred for 9 minutes and then left at room temperature for 1 hour to obtain solution C; solution C was centrifuged at 10,000 rpm for 3 minutes, the supernatant was discarded, and washing the precipitate with deionized water several times until the pH of the solution is neutral, and then drying the washed precipitate at 50°C for 24 hours to obtain the silver-graphene oxide nanomaterial;

[0070] (3) Grind the silver-graph...

Embodiment 3

[0075] (1) The gold electrode is used as the basic electrode, and Al of 1.0, 0.3 and 0.05 μm 2 o 3 The polishing powder is polished to a mirror surface, and then ultrasonicated in absolute ethanol for 8 minutes, N 2 blow dry;

[0076] (2) Dissolve 10 mg of graphene oxide in an aqueous solution and ultrasonically disperse it for 5 hours, then add 0.3 g of glucose to the above aqueous solution under stirring to obtain solution A; 3 ·H 2 O was slowly added to 5mL with a concentration of 0.27M AgNO 3 solution, solution B was obtained; solution B was slowly added to solution A, magnetically stirred for 10 minutes and then left at room temperature for 2 hours to obtain solution C; solution C was centrifuged at a speed of 13000 rpm for 6 minutes, the supernatant was discarded, and The precipitate was washed with deionized water several times until the pH of the solution was neutral, and then the washed precipitate was dried at 80° C. for 12 hours to obtain the silver-graphene oxi...

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Abstract

The invention provides an electrochemical immunosensor for detecting tiamulin antigen and a preparation method thereof. According to the invention, AgNPs is reduced to GO in situ to obtain the Ag-GO nano composite material, and the Ag-GO nano composite material shows good conductivity and oxidation-reduction activity. Perfluorosulfonic acid has excellent film-forming ability and high chemical stability and is used as a dispersing agent to obtain an Ag-GO-Nf composite solution, and the composite solution is used for modifying the surface of a basic electrode. Then, gold nanoparticles are electrodeposited on the surface of Ag-GO-Nf, and the effects of fixing an antibody and amplifying an electrochemical signal are achieved. The electrochemical immunosensor constructed by applying the method is simple, convenient, efficient and sensitive, has a wide detection range and a low detection limit when being used for detecting tiamulin antigens, and has good accuracy when being applied to detection of tiamulin residues in samples of animal-derived foods such as eggs, pork and pork livers.

Description

technical field [0001] The invention relates to the technical field of novel functional nanomaterials, immune molecules and biosensing detection, in particular to an electrochemical immunosensor for detecting tiamulin antigen and a preparation method thereof. Background technique [0002] Tiamulin (Tiamulin, TIA) is a semi-synthetic diterpene veterinary drug developed from pleuromutilin. It has good antibacterial activity against Gram-positive bacteria and Gram-negative bacteria. Ribosome peptidyl transferase center (peptidyl transferase center, PTC) binding to inhibit bacterial protein synthesis, mainly used for the treatment of chronic respiratory diseases of livestock and poultry. Adequate intake of TIA can not only effectively treat diseases, but also improve feed conversion rate. However, high-dose and / or continuous use of TIA can increase antibiotic resistance, and its residue in edible tissues of animals can lead to loss of appetite, fever, ataxia and other human hea...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48G01N33/577
CPCG01N27/3278G01N27/48G01N33/577
Inventor 王爱萍张改平有小娟周景明陈玉梅刘红亮丁培阳祁艳华李永欣张静马红芳
Owner 河南中泽生物工程有限公司
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