Application of a strain of Streptomyces lilacinus 2-1-2f-1 and its antibacterial substances in the control of vegetable soil-borne diseases

A technology for Streptomyces lavender and soil-borne diseases, applied in pest control, microbial-based methods, chemicals for biological control, etc., can solve the problem of unsatisfactory prevention and control of vegetable soil-borne diseases No problem, achieve low cost, convenient operation and good effect

Active Publication Date: 2022-07-26
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different sensitivities of pathogenic bacteria in different crops to pesticides, the control effect on vegetable soil-borne diseases in production is not ideal.

Method used

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  • Application of a strain of Streptomyces lilacinus 2-1-2f-1 and its antibacterial substances in the control of vegetable soil-borne diseases
  • Application of a strain of Streptomyces lilacinus 2-1-2f-1 and its antibacterial substances in the control of vegetable soil-borne diseases
  • Application of a strain of Streptomyces lilacinus 2-1-2f-1 and its antibacterial substances in the control of vegetable soil-borne diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Isolation of actinomycetes with strong antagonistic activity against Fusarium wilt of cucumber and tomato.

[0040] Soil samples were randomly collected from all parts of Jiangsu. The sampling method was to shovel the top 5 cm soil layer, take 50 g of fresh soil samples, store them in fresh-keeping bags, and record the sampling location and date;

[0041] Take 0.5g soil sample and add 1mL of water, shake it to make a soil suspension, and dilute it in a gradient of 10 3 ~10 5 times, take 100 μl of the dilution solution and spread it on the plate, and incubate it in a 28°C incubator for 48 hours;

[0042] Pick a single colony, streak it on Gao's No. 1 medium, purify it, and save it for later use;

[0043] Taking cucumber and tomato Fusarium wilt as the target bacteria, the antagonistic activity of the isolated strains was determined by the confrontation culture method, and the antibacterial bandwidth was determined by culturing at 24 ℃ for 4-5 days. The result...

Embodiment 2

[0044] Example 2: Classification and identification of strain 2-1-2F-1

[0045] (1) Morphological characteristics of strain 2-1-2F-1

[0046] Strain 2-1-2F-1 grows well on Gao's No. 1 solid medium, and both aerial mycelium and basal mycelium grow abundantly ( figure 2 Middle A), the colonies are circular, concentric rings, the hyphae are powdery, and the marginal hyphae are radial and multi-branched. With the differentiation and maturation of spore filaments and spores, the aerial hyphae gradually change from white to lavender grey( figure 2 Middle B), the mycelium in the base is white at the initial stage, and gradually becomes milky yellow at the later stage, and does not produce soluble pigment ( figure 2 middle C). Strain 2-1-2F-1 was observed under an optical microscope, and it was found that the spore filaments coexisted with straight and spiral shapes, with multiple linear spore filaments. figure 2 Medium D-E); the spore surface is smooth, round to oval, beaded,...

Embodiment 3

[0053] Example 3: Properties and composition analysis of the antibiotic active substances produced by the strain 2-1-2F-1

[0054] Strain 2-1-2F-1 was fermented in No. 4 fermentation broth at 30°C and 180r / min for 5 days, centrifuged at 5000r / min for 20min, took the supernatant, and added 4 times the volume of -20°C pre-cooled to the supernatant. Acetone, mix by inversion, overnight. Centrifuge at 5000 r / min for 30 min at 4 °C, remove the supernatant, and air dry at 0 °C. They were reconstituted with organic solvents of different polarities, and it was found that the antibiotics were insoluble in organic solvents such as acetonitrile, methanol, n-butanol, ethyl acetate, chloroform and petroleum ether, except that they were slightly soluble in dimethyl sulfoxide, but could be completely dissolved. in water ( Figure 4 ). The antibacterial substance was dissolved with PBS of 1 / 100 of the original volume, and the antibacterial ability of the antibacterial active substance was ...

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Abstract

Application of a strain of Streptomyces lilacinus 2-1-2F-1 and its antibacterial substances in the prevention and control of vegetable soil-borne diseases, preservation number CGMCC No.19768, the advantages and effects of the present invention: a stable source of natural raw materials, bacterial strains It comes from soil, and the screening method is simple; the raw materials of the fermentation medium, such as soybean meal powder and glucose, have low cost; the preparation of antibacterial active substances is simple and the operation is convenient;

Description

technical field [0001] The invention relates to biological control of plant diseases, belongs to the field of biological control research and application in the discipline of plant protection, and in particular relates to the application of a new Streptomyces lilacinus and its anti-biotic active substances. Background technique [0002] With the adjustment of industrial structure and the increase of people's demand for fresh vegetables, the planting area of ​​facility vegetables has increased year by year. High fertilizer, continuous cropping, and multi-stubble planting have provided the possibility for the accumulation of plant pathogens in the soil and the outbreak of soil-borne diseases. , Destructive soil-borne diseases occur from time to time in the vegetable producing areas of various facilities. At present, the prevention and control of soil-borne diseases of facility vegetables mainly relies on chemical agents. Excessive and excessive application of chemical agents ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00A01P1/00A01P21/00C12R1/465
CPCC12N1/20A01N63/28
Inventor 陈夕军吴冰越陈宸
Owner YANGZHOU UNIV
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