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Experimental method for determining anatomo-functional delimitation of sublaterodorsal tegmental nucleus (SLD)

A functional, borderline technology with applications in medicine and biology

Pending Publication Date: 2021-04-27
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for the delimitation of mouse SLD nuclei, the current general mouse brain atlas (The Mouse Brain in Stereotaxic Coordinates) written by Keith B.J. Franklin and George Paxinos [11] still refers to human anatomy when marking the nuclei Due to the anatomical differences between humans and animals, this annotation method has not been generally recognized by scholars in the sleep medicine research community

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  • Experimental method for determining anatomo-functional delimitation of sublaterodorsal tegmental nucleus (SLD)
  • Experimental method for determining anatomo-functional delimitation of sublaterodorsal tegmental nucleus (SLD)
  • Experimental method for determining anatomo-functional delimitation of sublaterodorsal tegmental nucleus (SLD)

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Embodiment Construction

[0052] Specific implementations of the present invention will be described in detail below, and each of the described specific implementations is not limiting and can be combined with each other.

[0053]The present invention adopts the means of reverse tracing of downstream nuclei combined with specific behavioral intervention to determine the anatomical functional boundary of SLD from two paths of anatomical fiber tracing and biological function manipulation, and the specific technical scheme is as follows:

[0054] 1. Directed injection of the downstream nucleus of SLD (GiV) reverse tracer of virus (anatomical labeling): By injecting neurons into the downstream nucleus of bilateral SLD - ventral giant cell reticular nucleus (GiV) - selective expression of fluorescent protein Retrotransport the virus vector to reversely label the distribution range of neurons (green fluorescent markers) that have fiber junctions with the downstream nucleus GiV in the SLD nucleus (see figure...

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Abstract

The invention relates to a method for positioning an animal SLD nucleus. The invention provides a method for determining the anatomo-functional delimitation of a sublaterodorsal tegmental nucleus (SLD) of a tested object. The method comprises the following steps that 1) a reverse transport virus vector is stereodirectionally injected into an SLD downstream nucleus abdominal side gigantocellular reticular nucleus (GiV), and under the action of a neuron alternative promoter, the reverse transport virus vector can specifically express a first marker in an upstream efferent neuron; 2) REM sleep deprivation and rebound experiments are conducted on a subject, and a functional active neuron is determined through a second marker, wherein the second marker is different from the first marker; and 3) the anatomo-functional delimitation of the SLD nucleus is determined according to the co-marker fusion range of the first marker and the second marker. According to the method, through ingenious combined application of nerve fiber projection and sleep physiology research methods, the anatomo-functional delimitation of the SLD nucleus can be determined visually and accurately.

Description

technical field [0001] The invention relates to the fields of medicine and biotechnology. In particular, the present invention relates to an experimental method for determining the anatomical and functional boundaries of the dorsolateral subdorlateral pontine tegmentum (SLD) in animals. Background technique [0002] One-third of a person's life is spent in sleep, and the research on sleep physiology and pathology has always been the frontier and hot spot of medical research. Among many sleep stages and phases, rapid eye movement sleep (REM) plays an important role in memory storage, organization, integration of learning ability and sleep maintenance. The sublaterodorsal tegmental nucleus (SLD) is the key nucleus involved in the initiation and maintenance of rapid eye movement (REM) sleep and the regulation of muscle atonia during the period, and determines its precise anatomical and functional boundaries ( Anatomo-functional delimitation) is undoubtedly of vital significan...

Claims

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Application Information

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IPC IPC(8): A61B90/00A61D1/00
CPCA61B90/39A61D1/00A61B2503/40A61B2090/395
Inventor 王坚沈岩郁文博黄志力沈博邬剑军孙一忞刘丰韬
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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