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Peptides having skin whitening activity and uses thereof

A skin whitening, serial number technology, applied in the field of peptides with skin whitening activity and its application, can solve the problems of freckle pigmentation, poor beauty, etc.

Active Publication Date: 2021-04-20
CAREGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the production of melanin is too much, it will lead to hyperpigmentation of chloasma, freckles, spots, etc., which will lead to bad results in beauty

Method used

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  • Peptides having skin whitening activity and uses thereof
  • Peptides having skin whitening activity and uses thereof
  • Peptides having skin whitening activity and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Embodiment 1, the synthesis of peptide

[0078] The peptide having the amino acid sequence of SEQ ID NO: 1 described in the following [Table 1] was synthesized by using an automatic peptide synthesizer (Milligen 9050, Millipore, USA), and the peptide was synthesized using C18 reverse phase high performance liquid chromatography (HighPerformance Liquid Chromatography; HPLC) (Waters Associates, USA) to purely isolate the synthesized peptides. ACQUITY UPLC BEH300 C18 (2.1 mm×100 mm, 1.7 μm, Waters Co, USA) was used as a column.

[0079] [Table 1]

[0080] serial number Sequence (5'→3') 1 LRKRKRRFWVL 2 FHFIPNW 3 WPFLRP

Embodiment 2

[0081] Example 2. Confirmation of the inhibitory effect on melanogenesis

[0082] Mouse melanoma cells, namely B16F10 cells, were mixed with 5×10 4 Cells / well density were seeded in 6-well plates and incubated for 16 hours. Next, after replacing the medium with a medium containing 2% serum, 200 ng / ml of α-melanocyte-stimulating hormones (α-MSH) was added to promote the production of melanin, and at the same time, 10 μM, 50 μM, 100 μM, or 200 μM of the peptide consisting of the amino acid sequence of SEQ ID NO: 1, 2, or 3 were added, respectively, and incubated for 72 hours. Next, the cultured cells were lysed using 1N NaOH, and the absorbance at 450 nm was measured. The untreated group (Con) was used as the control group, the group added with α-melanostimulating hormone alone was used as the negative control group, and arbutin (200 μM, 500 μM), which is known as a skin whitening agent, was added and á-Melanocyte-stimulating hormone group was used as positive control group. ...

Embodiment 3

[0084] Example 3, confirmation of the inhibitory effect on tyrosinase activity

[0085] Mouse melanoma cells, namely B16F10 cells, were mixed with 5×10 4 Cells / well density were seeded in 6-well plates and incubated for 16 hours. Next, replace the medium with a medium containing 2% serum, and add 200 ng / ml of α-MSH to the medium, and at the same time, add 1 μM, 10 μM, 20 μM, 40 μM, 60 μM, 80 μM, or 160 μM of the sequenced Peptides consisting of amino acid sequences No. 1, 2, or 3 were incubated for 72 hours. After the 6-well plate was placed on ice and washed with ice-cold PBS, 0.1 M sodium phosphate buffer (pH 6.8, lysis buffer) containing 1% triton X-100 was added thereto to lyse the cells. Then, the cells present in the well plate were scraped and collected in a 1.5 ml tube, and were vortexed and centrifuged at 15,000 rpm for 10 minutes to obtain a supernatant. Next, the protein in the supernatant was quantified and the supernatant was adjusted to include a certain amoun...

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Abstract

The present application relates to a peptide having skin whitening activity and uses thereof, and provides: a peptide comprising an amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3; a composition for skin whitening, comprising the peptide; a cosmetic composition comprising the composition for skin whitening; and a pharmaceutical composition for preventing or treating melanosis and hyperpigmentation diseases. The pharmaceutical composition comprises the composition for skin whitening.

Description

technical field [0001] The present application relates to peptides having skin lightening activity and uses thereof. Background technique [0002] In addition to differences based on race, region, gender, and age, factors that determine skin tone basically include melasma, freckles, and self-tanning through exposure to ultraviolet rays, as well as overall pigmentation, acne, scars, and keratin. Distribution status and blood circulation, stress, and health status, etc. Among the factors, pigmentation is known to be a major factor in determining skin color. [0003] Pigments that affect skin color include melanin, melanoids, carotene, hemoglobin, and carotenoids. The various colors of skin, hair, and eyes depend on these pigments. Among them, the most important pigment for determining skin color is melanin, specifically, skin color depends on the amount and distribution of melanin. Melanin is produced in cells called melanocytes (Melanocytes) under the epidermis, and moves ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K7/08A61K8/64A61P17/02A61Q19/02A61K38/00
CPCC07K7/06A61K38/00A61P17/02A61K8/64A61Q19/02C12N9/12Y02P20/55C07K7/08
Inventor 郑镕池金银美李应智J·D·金
Owner CAREGEN
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