Polypeptide mixture with oxidation resistance and DPP-IV inhibitory activity and preparation method of polypeptide mixture
A DPP-IV, anti-oxidation technology, applied in the field of food processing, can solve the problems of complex denaturation mechanism and difficulty in accurately preparing bioactive peptides
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[0041] The present invention also provides a preparation method of a polypeptide mixture having antioxidant and DPP-IV inhibitory activity, the preparation method comprising the following steps:
[0042] Step 1: subject the whey protein isolate solution with a concentration of 1% to ultra-high pressure treatment to obtain an ultra-high pressure isolated whey protein solution;
[0043] Step 2: adjust the pH of the ultra-high pressure separated whey protein solution described in step 1 to 2.0-3.0, then add pepsin and acid protease, heat and hydrolyze, inactivate the enzyme, centrifuge to take the supernatant, and freeze-dry after rotary evaporation to obtain crude peptide mixture;
[0044] Step 3: adjust the concentration of the crude polypeptide mixture to 1 mg / mL, and separate and purify it by gel filtration chromatography and RP-HPLC.
[0045] Specifically, in step 1, the pressure of the ultra-high pressure treatment is 200-600 MPa, and the time of the ultra-high pressure tr...
Embodiment 1
[0067] The present invention provides a polypeptide mixture with antioxidant and DPP-IV inhibitory activity. The specific source, amino acid sequence, site, ABTS free radical scavenging rate and DPP-IV inhibitory rate of the polypeptide mixture are shown in Table 1.
[0068] The amino acid sequence of the polypeptide mixture includes: HLVDEPQNLIK, DDQNPHSSN, DDEVDVDGTVEEDLGK.
[0069] The peptide mixture is derived from whey protein and has 14 peptides in total.
Embodiment 2
[0071] The present invention also provides a preparation method of a polypeptide mixture having antioxidant and DPP-IV inhibitory activity, the preparation method comprising the following steps:
[0072] Step 1: prepare a whey protein isolate solution with a concentration of 1% (w / w) with deionized water, divide the whey protein isolate solution into polyethylene bags and vacuum pack them, and place them in an ultra-high pressure container. Control the circulating water system in the ultra-high pressure vessel, so that the ultra-high pressure treatment is carried out under the conditions of the temperature in the vessel being 31°C, the ultra-high pressure boosting rate of 8.3MPa / min, the treatment pressure of 200-600MPa, and the treatment time of 5-30min to obtain ultra-high pressure Processed whey protein solution;
[0073] Step 2: Use 1mol / L hydrochloric acid to adjust the pH value of the whey protein isolate solution after the ultra-high pressure treatment in step 1 to 2.0-...
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