Method for simultaneously detecting multiple active ingredients in eucommia ulmoides by utilizing high performance liquid chromatography and wavelength switching technology

Abstract

The invention relates to a method for simultaneously detecting multiple active ingredients in eucommia ulmoides by utilizing a high performance liquid chromatographyand wavelength switching technology. The method comprises the following steps of: S1, treating eucommia ulmoides sample powder to be detected to prepare a sample solution; and S2, eluting the active ingredients in the sample solution by using a high performance liquid chromatography technology and taking the reference solution as a reference, and analyzing the active ingredients by using a wavelength switching technology. According to the method provided by the invention, the chromatographic peak pattern of the active ingredients in the eucommia ulmoides can be improved by selecting a proper mobile phase and utilizing a high performance liquid chromatography and wavelength switching technology, various characteristic ingredients in the eucommia ulmoides sample can be analyzed and detected more accurately and comprehensively, and the method has relatively high detection accuracy and sensitivity and high detection efficiency; and the quality of eucommia ulmoides resources can be better supervised and controlled, and the method has important significance in modernization and internationalization of traditional Chinese medicine.

Description

technical field [0001] The invention relates to the field of detection of plant active components, in particular to a method for simultaneously detecting multiple active components in Eucommia by using high-performance liquid chromatography wavelength switching technology. Background technique [0002] Eucommia ulmoides Oliv. is a perennial deciduous tree belonging to Eucommia ulmoides. It belongs to my country's unique resources. It is not only a traditional nourishing medicinal material in my country, but also a valuable economic crop. The cultivated area is about 3 million mu, accounting for more than 95% of the world's total. The resources are very rich, mainly distributed in Hunan, Henan, Shaanxi, Guizhou, Hubei and other places. It is listed as the top grade in "Shen Nong's Materia Medica", which can nourish the liver and kidney, strengthen the muscles and bones, and take it for a long time to strengthen the body and endure aging. Modern research has proved that the ch...

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Problems solved by technology

However, the types of compounds involved in simultaneous detection in the current literature are limited, and the maximum absorption wavelengths of different compounds are also different. When detecting multiple compound components at the same time, the unreasonable detection wavelength often directly leads to the corresponding value of the compound chromatographic peak; In addition, due to the similar polarity of some characteristic compounds in Eucommia ulmoides, using the conventional isocratic elution method, the separation effect of the chromatographic peaks is not good.

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