Cosmetic composition containing industrial hemp leaf extract and preparation method thereof
A cosmetic composition and extract technology are applied in the field of essence or gel containing industrial hemp leaf extract and its preparation, which can solve the problems of insecurity and poor use effect.
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Embodiment 1
[0141] Inhibition experiments on tyrosinase activity of three plant extracts of industrial hemp leaves, licorice, and Tribulus terrestris: tyrosinase can catalyze the substrate tyrosine to generate dopaquinone, and then undergo a series of reactions to finally generate melanin, tyrosine Enzyme-catalyzed reaction is considered to be the rate-limiting step of melanin production, so substances that inhibit tyrosinase activity are considered to have potential whitening activity. Dopaquinone is a colored substance with a characteristic absorption peak at 470nm. The amount of dopaquinone generated is directly proportional to the activity of the enzyme. Therefore, the amount of dopaquinone generated in the system can be measured by a spectrophotometer to reflect the enzyme activity. size.
[0142] Main reagents:
[0143] Tyrosinase (Mushroom Source) (Shanghai Yihe Biotechnology Co., Ltd., ≥500U / mg); L-Tyrosine (Aladdin, 99.0%~101.0%); α-Arbutin (DSM, ≥98 %); disodium hydrogen phosp...
Embodiment 2
[0155] Essence DPPH free radical scavenging experiment: DPPH free radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to pairing with its single electron, and its fading degree has a quantitative relationship with the number of electrons it accepts, so a spectrophotometer can be used for rapid quantitative analysis.
[0156] Main reagents: DPPH (Shanghai Yuanye Biotechnology Co., Ltd., ≥97%); ascorbic acid (Vc), and methanol are of domestic analytical grade.
[0157] Prepare the essence lotion sample according to the following raw material composition and method:
[0158] Sample No. 5:
[0159] Component A: 0.5 parts of rhamnose, 2 parts of trehalose, 2 parts of nicotinamide, 0.2 parts of sodium hyaluronate, 30 parts of deionized water;
[0160] Component B: 0.3 parts of carbomer, 0.3 parts of polyacrylate crosslinked polymer-6, 8 parts of deion...
Embodiment 3
[0180] Test the skin brightness of the test subjects: the volunteers applied the blank essence samples containing the No. 7 essence sample in Example 2 and the blank essence sample that did not contain the plant extract in the No. 7 sample (hereinafter referred to as No. 0 sample), 5 subjects were continuously tested for 6 weeks, and the ITA values of the subjects were tested in the 2nd, 4th, and 6th weeks of the subjects' use. The specific test results are shown in Table 3-5.
[0181] Which contains the average rate of change of the No. 7 essence sample of the present invention and the No. 0 blank essence sample ITA value see figure 1 .
[0182] Table 3 ITA test value of subjects in the second week
[0183]
[0184] Table 4 ITA test value of subjects in the 4th week
[0185]
[0186] Table 5 ITA test value of subjects in the 6th week
[0187]
[0188] Analyze Table 3-5 and figure 1 It can be seen that the ITA value of the skin of the test subjects who use the ...
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