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Tissue culture method of habenaria rhodocheila

A technology of orange-yellow phoenix flower and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low natural reproduction coefficient, low germination rate in the field, and few seedlings, so as to ensure the stability of the population Sex, increase the survival rate of proliferation and differentiation, and improve the effect of proliferation efficiency

Active Publication Date: 2021-04-13
惠州市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the reproductive ability of the wild population of the orange-yellow phoenix flower is low, the seeds of the orange-yellow phoenix flower are small, the germination rate in the field is extremely low, and there are few seedlings, so that the seeds of the orange-yellow phoenix flower make little contribution to the renewal and maintenance of the population, and the natural reproduction coefficient Low, when the mother plant of the orange-yellow phoenix flower is infringed, it will directly affect the stability of the orange-yellow phoenix flower population, making the smaller populations of the orange-yellow phoenix flower likely to be extinct at any time

Method used

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  • Tissue culture method of habenaria rhodocheila
  • Tissue culture method of habenaria rhodocheila
  • Tissue culture method of habenaria rhodocheila

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086](1) Exterior implant disinfection

[0087]First, use the surgery to cut the upper part of the orange-yellow jade phoenix flowers, using brush under water, use brush to carefully clean the soil and impurities, then rinse under water under water, then put it in ultrasonic cleaning machine with 0.2% (W / V) Sterilization was 30 min, and finally washed 5 times with sterile water. After the percentage of alcohol disinfection of 75% of the ultra-net table, it was used to sterilize the mercury and sterilized by 0.1% (w / v), then washed 5 times with a sterile water, and sterilized paper spare.

[0088](2) Seedling breeding

[0089]The root stalk treated in (1) was cut out of the skin after sterilization, and then the root stem cutting of the outer skin was 0.5 cm, a width of 0.5 cm, and a square sheet having a thickness of 0.2 cm inoculated into induced medium (1 / 2MS + 6-BA0.5mg / L + NaA0.1mg / L + AC0.2% + 30GL-1Sucrose + 5.0GL-1Culture in agar), cultured 24D to produce original bulbs, 35...

Embodiment 2

[0097](1) Exterior implant disinfection

[0098]First, use the surgery to cut the upper part of the orange yellow jade flowers, use hair brush under the water to carefully clean the soil and impurities, then rinse under the water under water, then put it in ultrasonic cleaning machine with 0.2% sterilization net cleaning. 30min, finally washed 5 times with sterile water. After using an alcohol disinfection of 75% in the ultra-net table, it was fired with 0.1% (w / v) by mercury and mercury and disinfection, then washed 5 times with sterile water, and dry it with aseptic filter paper. spare.

[0099](2) Seedling breeding

[0100]The root stalk treated in (1) was cut out of the skin after sterilization, and then the root stem cutting of the outer skin was 0.5 cm, a width of 0.5 cm, and a square sheet having a thickness of 0.2 cm inoculated into induced medium (1 / 2MS + 6-ba 1.0mg / L + NaA0.5mg / L + AC0.2% + 30GL-1Sucrose + 5.0GL-1Culture in agar), cultured 15 days to produce original bulbs,...

Embodiment 3

[0108](1) Exterior implant disinfection

[0109]First use the bacteria to spray the orange-yellow jade flowers. After seven days, use the surgery to cut the upper part of the orange yellow jade flowers, using brush under the water, carefully cleans the soil and impurities, then rinse under water 4h After the ultrasonic cleaning machine was placed in an ultrasonic cleaning machine for 30 min, and finally washed 5 times with sterile water. After using a volume of alcohol disinfection of 75% in the ultra-net table, it was 1.0.1% (w / v) lifting and mercury disinfection was 15 min, and then washed 5 times with sterile water, and dry it with aseptic filter paper. spare.

[0110](2) Seedling breeding

[0111]The root stalk treated in (1) was cut out of the skin after sterilization, and then the root stem cutting of the outer skin was 0.5 cm, a width of 0.5 cm, and a square sheet having a thickness of 0.2 cm inoculated into induced medium (1 / 2MS + 6-ba 1.5mg / l + NaA1.0mg / L + AC0.2% + 30GL-1Su...

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Abstract

The invention provides a tissue culture method of habenaria rhodocheila. The tissue culture method of the habenaria rhodocheila comprises the following steps: obtaining an explant of the habenaria rhodocheila; carrying out disinfection treatment on the explant; carrying out skin scraping treatment on the explant subjected to disinfection treatment; carrying out inoculated culture operation on the explant subjected to the skin scraping treatment to obtain explant cluster buds; carrying out proliferation and rooting culture operation on the explant cluster buds ti=o obtain sterile bottle seedlings; and carrying out domestication and cultivation operation on the sterile bottle seedlings to obtain the habenaria rhodocheila. According to the tissue culture method of the habenaria rhodocheila, the proliferation efficiency of the habenaria rhodocheila is improved, and the population quantity stability of the habenaria rhodocheila is ensured.

Description

Technical field[0001]The present invention relates to the field of tissue culture techniques, and more particularly to tissue culture methods of orange-yellow jade.Background technique[0002]Orange Huang Yufeng flower Lanka Yufeng flower is a perennial plants, producing Jiangxi, Fujian, Hunan, Guangdong, etc., is born with 300 meters to 1500 meters above sea level or the guret in the guret in the earth or rock, orange yellow. The jade-style is elegant, the flower is beautiful, the color is novel, the flowery is beautiful, and it is very good. In addition to watching value, orange yellow jade phoenix flowers have a certain effect on treating pulmonary heat cough, sore swelling, and falling injury, with liquid value of heat and detoxification, blood circulation and pain relief.[0003]In our country, rarely cultivate orange yellow jade phoenix flowers, people are over picking wild resources, making orange yellow jade info. "The Convention on the International Trade of Endangered Wildlife...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40
Inventor 刘德浩郑洲翔廖文莉陈智涛邓仿东舒夏竺阳艳萍吴宝宏张鑫张展鹏周建芬刘健
Owner 惠州市林业科学研究所
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