1,3-beta-D glucan derivative, kit, preparation method and method for determining content of 1,3-beta-D glucan

Abstract

The invention discloses a 1,3-beta-D glucan derivative, a kit, a preparation method and a method for determining the content of 1,3-beta-D glucan. The invention provides a 1,3-beta-D glucan magnetic particle chemiluminiscence immunoassay kit which combines a magnetic particle separation technology, a biotin-avidin amplification technology and a chemiluminiscence analysis technology. Meanwhile, a biotinylated 1,3-beta-D glucan antigen derivative and an enzyme-labeled 1,3-beta-D glucan antigen derivative are prepared from the 1,3-beta-D glucan antigen derivative, so that two systems (1, the biotinylated 1,3-beta-D glucan antigen derivative plus an antibody enzyme-labeled reagent and plus a calibration solution; 2, the enzyme-labeled 1,3-beta-D glucan antigen derivative plus a biotinylated 1,3-beta-D glucan mouse monoclonal antibody and plus calibration liquid) are constructed, and the 1,3-beta-D glucan in a sample can be tested, so that different enterprises have more flexible selection when developing the kit, and the kit can be realized by only preparing the intermediate, namely, the 1,3-beta-D glucan antigen derivative.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and more specifically relates to a 1,3-beta-D glucan derivative and a preparation method thereof and the determination of the content of 1,3-beta-D glucan by using magnetic particle chemiluminescence method Kit and preparation method thereof. Background technique [0002] 1,3-β-D glucan is a kind of polysaccharide, widely exists in fungal cell walls except zygomycetes, accounts for more than 50% of fungal cell wall components, and is a unique component on fungal cell walls. After the fungus enters the blood or deep tissues of the human body, 1,3-β-D glucan can be released from the cell wall through phagocytosis and digestion by phagocytic cells, increasing the content in blood and other body fluids; while in superficial fungal infections , 1,3-β-D glucan is not released, so its content in body fluids does not increase, and the detection of 1,3-β-D glucan in blood or non-blood samples can be ...

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems in the background technology, the existing 1,3-β-D glucan kits cannot be used for both solid-phase coupling and luminescence-labeled 1,3-β-D glucan Defects of derivatives, the biggest feature of this technical solution is to provide a 1,3-β-D glucan antigen derivative and its preparation method, through the preparation of derivatives, 1,3-β -D glucan antigen is indirectly connected with biotin or enzyme, which solves the key problem of the development of this project, that is, the coupling problem, and can conveniently establish a magnetic particle enzymatic chemiluminescent system of 1,3-β-D glucan

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