Method for obtaining industrial hemp unisexual plant tissue culture seedlings

Abstract

The invention discloses a method for obtaining industrial hemp unisexual plant tissue culture seedlings. The method comprises the following steps of (1) selecting and sterilizing explants, namely taking industrial hemp seeds, washing with water, and disinfecting; (2) culturing sterilized materials, namely placing the inoculated explants in a culture room, carrying out dark culture, then carrying out normal culture until new buds grow out, taking a new bud germinated from each seed as an individual strain marker, and transferring the individual strain markers to a culture medium for subsequent culture until an industrial hemp sterile system is established; and (3) obtaining unisexual sterile plants, namely inoculating the new buds cultured in the step (2) into a differential medium, wherein the formula of the differential medium comprises MS+1.0 mg.L<-1>6-BA+0.3 g.L<-1> activated carbon+25 g.L<-1> sucrose+5 g.L<-1> agar powder (the gel strength of the agar powder is 1300g / cm<2>). Compared with a method for observing and screening unisexual plants through a field, the method has the advantages of shortening the time by 60%-80% and greatly improving the selection efficiency.

Description

technical field [0001] The invention belongs to the technical field of plant artificial cultivation, and in particular relates to a method for obtaining industrial hemp monosexual plant tissue culture seedlings. Background technique [0002] Cannabis for industrial use is also known as hemp, which belongs to the Cannabis family Cannabis genus, an annual herb, with tall plants and dioecious plants. Native to central Asia, it is one of the earliest crops planted by humans. Industrial hemp is full of treasures and can be extended from agricultural planting to textiles, clothing, papermaking, military supplies, chemicals, new building materials, bio-energy, food health care, medicine, feed, etc. More than a dozen industrial chains have spread all over the world. [0003] Since industrial hemp is a dioecious plant, artificially cultivated industrial hemp varieties are also dioecious, and the ratio of female plants to male plants is also close to 1:1. However, according to exist...

AI Technical Summary

Problems solved by technology

However, according to existing studies, the fiber yield and quality of industrial hemp male plants are superior to those of female plants. Therefore, when harvesting fibers, it is advisable to keep more male plants, and the content of CBD in female plant flowers and leaves is significantly higher than that of male plants. If you want to use For CBD, the male plant needs to be removed during the planting process, because the CBD content in the flowers and leaves of the female plant will decrease a lot after pollination

At present, there are relatively few reports on the tissue culture of industrial hemp, and there is no relevant report on the method of using tissue culture technology to obtain unisexual tissue cultured seedlings. Due to the use of female plants for the purpose of medicinal industrial hemp, when the production is relatively restricted, facing the domestic planting demand of hundreds of thousands of acres, a seedling method that can obtain industrial hemp unisexual plants has been developed. It is of great value in actual production

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