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A method for producing fish collagen peptides from tilapia scale raw materials

A technology for fish collagen peptide and fish scale collagen, which is applied in the field of fish collagen peptide preparation, can solve the problems of complex extraction product components, troublesome purification and poor uniformity, etc., and achieves the improvement of resource utilization, yield, and molecular weight concentration. Effect

Active Publication Date: 2022-03-18
山东恒鑫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen peptides are extracted by acid and alkali methods. The molecular weight distribution of the product is wide and the uniformity is poor. In order to prepare collagen peptides with a small molecular weight, a large amount of acid and alkali must be consumed in the extraction process, and the extracted product components are very complicated. Subsequent purification deal with trouble

Method used

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  • A method for producing fish collagen peptides from tilapia scale raw materials
  • A method for producing fish collagen peptides from tilapia scale raw materials

Examples

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Effect test

Embodiment 1

[0030] A method for producing fish collagen peptides from tilapia scale raw materials is prepared by the following steps:

[0031] 1) Pretreatment: add the cleaned tilapia scales to 0.05mol / L NaOH solution, stir for 8-10 hours, and control the water; then soak in 0.04mol / L HCl solution for 2 hours, and control the water; Finally, add it to the compound solution and soak for 50 minutes, wash with water until neutral, and obtain pretreated fish scales;

[0032] 2) Activation treatment: add the pretreated fish scales to the activation solution, soak at 35°C for 4 hours, raise the temperature to 90°C, keep for 5 minutes, naturally cool to room temperature, wash with water, control the moisture, dry, crush and pass through an 80-mesh sieve , to obtain activated fish scale powder;

[0033] 3) Extraction of collagen: mix activated fish scale powder, acid protease and deionized water, extract at 45±2°C and pH=6 for 3 hours, centrifuge, collect supernatant, and obtain primary collagen...

Embodiment 2

[0043] A method for producing fish collagen peptides from tilapia scale raw materials is prepared by the following steps:

[0044] 1) Pretreatment: add the cleaned tilapia scales to 0.05mol / L NaOH solution, stir for 8-10 hours, and control the water; then soak in 0.04mol / L HCl solution for 2 hours, and control the water; Finally, add it to the compound solution and soak for 30 minutes, wash with water until neutral, and obtain pretreated fish scales;

[0045] 2) Activation treatment: add the pretreated fish scales to the activation solution, soak at 45°C for 2 hours, raise the temperature to 95°C, keep for 3 minutes, naturally cool to room temperature, wash with water, control the moisture, dry, crush and pass through a 100-mesh sieve , to obtain activated fish scale powder;

[0046] 3) Extraction of collagen: mix activated fish scale powder, acid protease and deionized water, extract at 45±2°C, pH=6 for 1 hour, centrifuge, collect supernatant, and obtain primary collagen liq...

Embodiment 3

[0056] A method for producing fish collagen peptides from tilapia scale raw materials is prepared by the following steps:

[0057] 1) Pretreatment: add the cleaned tilapia scales to 0.05mol / L NaOH solution, stir for 8-10 hours, and control the water; then soak in 0.04mol / L HCl solution for 2 hours, and control the water; Finally, add it to the compound solution and soak for 40 minutes, wash with water until neutral, and obtain pretreated fish scales;

[0058] 2) Activation treatment: Add the pretreated fish scales to the activation solution, soak at 40°C for 3 hours, raise the temperature to 92°C, keep for 4 minutes, naturally cool to room temperature, wash with water, control the moisture, dry, crush and pass through a 90-mesh sieve , to obtain activated fish scale powder;

[0059] 3) Extraction of collagen: mix activated fish scale powder, acid protease and deionized water, extract at 45±2°C, pH=6 for 2 hours, centrifuge, collect supernatant, and obtain primary collagen liq...

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Abstract

The invention discloses a method for producing fish collagen peptides from tilapia scale raw materials, which is prepared by the following steps: pretreatment; activation treatment; collagen extraction; collagen peptide extraction. The method of the present invention can completely enzymatically hydrolyze collagen, the molecular weight of the enzymolyzed product is small and concentrated, the amino acid content of the enzymatically hydrolyzed product is low, and the obtained collagen peptide is a white odorless powder with high yield and the ash content is less than 0.6g / 100g, which not only guarantees the The high yield of protein peptide, and the molecular weight of the prepared collagen peptide is small and concentrated, the finished product is delicate, and the water solubility is good, which is conducive to large-scale production.

Description

technical field [0001] The invention relates to the field of preparation of fish collagen peptides, in particular to a method for producing fish collagen peptides from tilapia scale raw materials. Background technique [0002] Collagen (Collagen) is a kind of macromolecular protein, which is the protein with the most content in animals, accounting for about 25-33% of human protein, equivalent to 6% of human body weight, and has the functions of supporting organs and protecting the body. Collagen is a helical fibrous protein twisted by 3 peptide chains, widely distributed in connective tissue, skin, bone, visceral interstitium, muscle cavity, ligament, sclera and other parts. 70% to 80% of the organic matter in the bone is collagen. When the bone is formed, sufficient collagen fibers must first be synthesized to form the framework of the bone. Therefore, some people call collagen the bone in the bone. Collagen is also the main component in the skin, accounting for more than...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/34C07K1/14
CPCC12P21/06C07K14/78
Inventor 张格李江王辉林冰莹王璇
Owner 山东恒鑫生物科技有限公司
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