Temperature control method of CO2 incubator

A temperature control method and incubator technology, applied in the field of temperature control of CO2 incubators, can solve problems such as inability to accurately control insects, and achieve the effect of avoiding temperature overshoot

Active Publication Date: 2021-04-02
冰山松洋生物科技(大连)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current CO 2 The incubator is used at 37°C, and it is used to cultivate human and mammalian cells. It cannot precisely control the temperature environment required for cell culture of insects, birds, cold-blooded animals, etc.

Method used

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  • Temperature control method of CO2 incubator
  • Temperature control method of CO2 incubator
  • Temperature control method of CO2 incubator

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Embodiment Construction

[0032] In order to make the technical solutions and advantages of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the drawings in the embodiments of the present invention:

[0033] Such as figure 1 shown as a CO 2 The temperature control method of the incubator, the method calculates the floating control parameters through the ambient temperature and the set temperature, and uses the temperature control algorithm to control the output power of the heating element, so that the CO 2 When the incubator is in the environment of 15-35°C and the set temperature is 30-50°C, the control error can be kept at ≤±0.2°C. When the set value is 37°C and the ambient temperature is 23°C, the control accuracy can be guaranteed At ≤±0.1°C. Among them, St is the set temperature, t is the temperature in the box, EL is the lower limit value of the control output, EH is the upper li...

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Abstract

The invention discloses a temperature control method of a CO2 incubator. The temperature control method comprises the following steps that: a temperature control deviation value er is calculated according to a set temperature st and a real-time temperature t in the CO2 incubator; and the output power of a heating element is controlled by adopting the CO2 incubator temperature control method, and the temperature t in the CO2 incubator is controlled within an accurate range. According to the method, temperature overshoot is effectively avoided, the stability and uniformity of the box temperatureare effectively controlled, it is guaranteed that different cells can be cultured under different environment conditions, the control error of the CO2 incubator can be kept smaller than or equal to + / -0.2 DEG C in the environment of 15-35 DEG C and at the set temperature of 30-50 DEG C, and when the set value is 37 DEG C and the environment temperature is 23 DEG C, the control precision can be ensured to be less than or equal to + / -0.1 DEG C.

Description

technical field [0001] The invention relates to the field of incubator control, in particular to a CO 2 The temperature control method of the incubator. Background technique [0002] The carbon dioxide incubator is a device for culturing cells / tissues in vitro by simulating a growth environment similar to cells / tissues in organisms in the incubator. It is an advanced instrument for cell, tissue, and bacterial culture. It is the key equipment necessary for immunology, oncology, genetics and bioengineering. [0003] Research and production in scientific fields such as microbiology, medicine, pharmaceuticals, environmental protection, food, animal husbandry, etc. Commonly used in cell kinetics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, cultivation of hybridoma cells to produce antibodies, in vitro fertilization (IVF), stem cells, tissues In research fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G05D23/32
CPCG05D23/32
Inventor 刘发柱邱长义孙晓雪于吉帅战雷刘锦洋李梦莹郑建徐桂杰郑永权王佳宁刘强
Owner 冰山松洋生物科技(大连)有限公司
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