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Pymetrozine degrading bacterium IUMR B53 and application thereof

A technology of pymetrozine and degrading bacteria, applied in the application, bacteria, biocide and other directions, can solve the problem that the degrading strain of pymetrozine is rarely reported, etc.

Active Publication Date: 2021-04-02
CHANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on pymetrozine-degrading strains, and further research is needed, which has great practical significance for eliminating pymetrozine pollution in the environment, minimizing food safety hazards, and purifying the environment.

Method used

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  • Pymetrozine degrading bacterium IUMR B53 and application thereof
  • Pymetrozine degrading bacterium IUMR B53 and application thereof
  • Pymetrozine degrading bacterium IUMR B53 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Cultivate the pymetrozine-degrading bacteria IUMR B53 in LB liquid medium, collect the bacteria by centrifugation, resuspend with sterile water and dilute to OD 600 =1, inoculate 1mL of the selected strain into the basic liquid inorganic salt medium containing 100.0mg / L pymetrozine, place it in a constant temperature shaker at 180r / min, and cultivate it at 35°C for 30h, the pymetrozine-degrading bacteria IUMR B53 has Visible growth.

Embodiment 2

[0062] The pymetrozine-degrading bacteria IUMR B53 was inoculated in 100 mL of sterile MSM at an inoculum amount of 1%, and pymetrozine was added to a final concentration of 100 mg / L, and cultured at 35°C and 180 r / min for 30 hours to determine the pymetrozine The degradation rate is 91.33% (see figure 2 ).

Embodiment 3

[0064] The pymetrozine-degrading bacteria IUMR B53 was inoculated in 100mL of pymetrozine-containing wastewater according to the inoculation amount of 1%. The content of pymetrozine in the wastewater was determined to be 58 mg / L, and cultured at 35°C and 180r / min. 30h, measure the degradation rate of pymetrozine: 77.63% (see image 3 ).

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Abstract

The invention discloses a pymetrozine degrading bacterium IUMR B53 and an application thereof, belongs to the technical field of biology, and provides the pymetrozine degrading bacterium and the application thereof. Microorganisms capable of degrading pymetrozine are separated and screened from specific environmental conditions according to the characteristics of the pymetrozine by virtue of a microbial technical means, and experiments prove that the pymetrozine degrading bacterium IUMRB53 provided by the invention has very high pymetrozine degrading activity, the pymetrozine degrading rate can reach 45-90%, and the pymetrozine degrading bacterium IUMRB53 provided by the invention can be applied to pymetrozine degradation in pesticide polluted environment, and has a very good application value in reducing environmental pollution.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a pymetrozine-degrading bacterium IUMR B53 and its application. Background technique [0002] With the continuous improvement of people's living standards, the quality and safety of agricultural products has become a hot spot of concern. The use of pesticides in the production and planting of agricultural products has become an indisputable fact. Pesticides are used to kill organisms such as insects that harm crop growth. The use of pesticides has protected the growth of crops to a certain extent and increased crop yields, but the long-term use of pesticides in large quantities has not only brought huge pressure to the ecological environment, but also brought safety hazards in terms of food safety and other aspects. Pymetrozine, as a new pyridine heterocyclic insecticide, has the characteristics of high efficiency, low toxicity, and high selectivity. Its preparations can be used to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34A62D3/02A01N25/32C12R1/22C02F101/38A62D101/04A62D101/28
CPCC12N1/20B09C1/10C02F3/34A62D3/02A01N25/32C02F2101/306C02F2101/38A62D2101/04A62D2101/28
Inventor 牛东泽张晋任建军孙如梦李春雨呼和涛力郑亚斌王永江魏文亮
Owner CHANGZHOU UNIV
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