Isolation and application of an oil-degrading bacterium kosakonia cowanii IUMR B67

A technology for degrading bacteria and oil, which is applied in the biological field to achieve high oil degradation characteristics and avoid secondary pollution

Active Publication Date: 2022-05-17
CHANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports of oil-degrading strains, and further research is needed, which has great practical significance for eliminating the pollution of waste oil in the environment, shortening the fermentation cycle of food waste, and purifying the environment.

Method used

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  • Isolation and application of an oil-degrading bacterium kosakonia cowanii IUMR B67

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Cultivate the oil-degrading bacteria IUMR B67 in LB liquid medium, collect the bacteria by centrifugation, resuspend with sterile water, and dilute to OD 600 =1, inoculate 1mL of the selected strain into 100.0mL basic liquid inorganic salt medium containing 100μL Tween 80 and 1mL soybean oil, place it in a constant temperature shaker at 180r / min, and cultivate it at 37°C. The degradation rates are respectively: 71.2% and 95.5%; the oil degradation efficiency is obvious.

Embodiment 2

[0066] The oil-degrading bacteria IUMRB67 was inoculated into 100 grams of sterilized restaurant leftovers at an inoculum amount of 1%. The oil content in the food waste was determined to be 6%, and cultured at 37°C and 180r / min for 72h , Determination of oil degradation rate: 19.25%.

Embodiment 3

[0068] The oil-degrading bacteria IUMRB67 was inoculated in 100mL of sterilized oil-containing wastewater at an inoculum amount of 1%. The oil content of the wastewater was determined to be 0.8%, and cultured at 37°C and 180r / min for 72h to measure the oil degradation rate. For: 33.33%.

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Abstract

The invention discloses an oil-degrading bacterium Kosakonia cowaniIUMRB67 and an application thereof, belonging to the field of biotechnology. Experiments have proved that the oil-degrading bacteria IUMRB67 of the present invention can survive in an environment where oil is the only carbon source, and has high oil-degrading activity. The grease-degrading bacteria IUMRB67 provided by the invention can be applied to grease degradation in polluted environments, and has good application value in kitchen waste treatment and environmental restoration.

Description

technical field [0001] The invention belongs to the field of biological technology, in particular to an oil-degrading bacterium and its application in the field of kitchen waste or waste water oil degradation. Background technique [0002] With the increase of the urban population and the improvement of the quality of life, the total amount of food waste and the oil content are also increasing accordingly. Because the food waste is rich in nutrition, it is easy to rot and deteriorate if it is not handled properly, which will pollute the environment and endanger human health. Waste oil from food waste contains a large amount of pathogenic bacteria and toxins. If it is directly fed to pigs and other livestock without treatment, it is easy to spread pathogenic bacteria and toxins to humans; After the oil enters the soil, it will reduce the air permeability and water permeability of the soil, hinder the absorption and respiration of the plant root system, cause the root system t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01C02F101/34
CPCC12N1/20C12N1/02C02F3/34C02F2101/34C12R2001/01C12N1/205
Inventor 任建军李春雨张晋牛东泽沈张磊黄煦
Owner CHANGZHOU UNIV
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