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Pymetrozine degrading bacterium RJJ-1 and application thereof

A technology of RJJ-1 and pymetrozine, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of weak cytotoxicity and high toxicity, and achieve the effect of avoiding secondary pollution

Active Publication Date: 2021-03-30
CHANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pymetrozine has been reported to be highly toxic to aquatic organisms and has both genotoxic and weak cytotoxic effects on human peripheral blood lymphocytes

Method used

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  • Pymetrozine degrading bacterium RJJ-1 and application thereof
  • Pymetrozine degrading bacterium RJJ-1 and application thereof
  • Pymetrozine degrading bacterium RJJ-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Cultivate the pymetrozine-degrading bacteria RJJ-1 in LB liquid medium, collect the bacteria by centrifugation, resuspend with sterile water, and dilute to OD 600 =1, inoculate 1mL of the selected strain into the basic liquid inorganic salt medium containing 100.0mg / L pymetrozine, place it in a constant temperature shaker at 180r / min, and culture at 37°C for 30h, the pymetrozine-degrading bacteria RJJ-1 There is obvious growth, and the degradation efficiency of pymetrozine is higher.

Embodiment 2

[0070] The pymetrozine-degrading bacteria RJJ-1 was inoculated in 100 mL of sterile MSM at an inoculation amount of 1%, and pymetrozine was added to a final concentration of 100 mg / L, and cultured at 37°C and 180 r / min for 30 hours, and the pyrmetrozine was measured. Metrozine degradation rate is 86.34% (see figure 2 ).

Embodiment 3

[0072] The pymetrozine-degrading bacteria RJJ-1 was inoculated in 100mL of pymetrozine-containing wastewater at an inoculation amount of 1%. The content of pymetrozine in the wastewater was determined to be 60mg / L. Under the conditions of 37°C and 180r / min Cultivate for 30h, measure the degradation rate of pymetrozine: 79.33% (see image 3 ).

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Abstract

The invention discloses a pymetrozine degrading bacterium RJJ-1 and an application thereof, belongs to the technical field of biology. Experiments prove that the pymetrozine degrading bacterium RJJ-1can survive in an environment taking pymetrozine as a unique carbon source, and has high pymetrozine degrading activity. The pymetrozine degrading bacterium RJJ-1 provided by the invention can be applied to degradation of pymetrozine pesticides polluting the environment, and has good application value in the aspects of soil pollution treatment and environmental remediation.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a pymetrozine-degrading bacterium RJJ-1 and its application. Background technique [0002] With the increase of population pressure, the global demand for food is gradually increasing. As an agricultural means to effectively reduce crop losses and improve the quality of agricultural products, pesticides and fertilizers are favored by people, but at the same time, pesticide residue pollution also affects the ecological environment. serious harm to human health. Pymetrozine is a pyridine nitrogen methylamine insecticide, because of its excellent insecticidal effect, it is widely used in the control of pests such as whitefly, leafhopper and cotton aphid in crops. However, the irrational and excessive use of chemical pesticides in the process of preventing and controlling crop diseases and insect pests has resulted in higher and higher pesticide residues in agricultural products. Thirt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C12R1/385C02F101/38C02F101/34
CPCC12N1/20C12N1/02C02F3/34B09C1/10C02F2101/306C02F2101/34C02F2101/38B09C2101/00Y02W10/10
Inventor 牛东泽张晋任建军李春雨王珍珠呼和涛力郑亚斌王永江魏文亮
Owner CHANGZHOU UNIV
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