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Pymetrozine degrading bacterium IURM B56 and application thereof

A technology of pymetrozine and degrading bacteria, applied in the biological field to achieve the effect of avoiding secondary pollution

Active Publication Date: 2021-03-16
CHANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pymetrozine-degrading strains are rarely reported, and the isolation and screening of pymetrozine-degrading bacteria has great practical significance for eliminating pymetrozine pollution in the environment, and can ensure food safety and purify the ecological environment

Method used

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  • Pymetrozine degrading bacterium IURM B56 and application thereof
  • Pymetrozine degrading bacterium IURM B56 and application thereof
  • Pymetrozine degrading bacterium IURM B56 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067]The pyrid degradation of the pyidone is cultured in LB liquid medium, centrifugally collecting the bacteria, hovering with sterile water, and dilute to OD600= 1, in the inorganic salt medium containing 1ml to 100 ml of 100.0 mg / L piperidone in the inorganic salt medium containing 100.0 mg / L pyrid, 180 r / min, 37 ° C culture, and the pyrid degradation of bacteria is obvious. Growth, highly degradation efficiency of piirone.

Embodiment 2

[0069]The piperidone degradation of the pyrene is inoculated into 100 mL of a sterile MSM in a 1% inoculation amount, and a pyrid is added to the final concentration of 100 mg / L, placed under 37 ° C, 180R / min, and cultured under the conditions of 30 h, and determines pyrid Ketone degradation rate is 92.58% (seefigure 2 ).

Embodiment 3

[0071]The pyrid degradation of the pyidone is inoculated in a wastewater of 100 ml of pyidone, and the content of the pyidone containing the wastewater is 58 mg / L, and cultured under 37 ° C, 180R / min. 30h, the degradation rate of pypatum is 71% (seeimage 3 ).

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Abstract

The invention discloses a pymetrozine degrading bacterium IURM B56 and application thereof, and belongs to the technical field of biology. Experiments prove that the pymetrozine degrading bacterium IURM B56 can survive in an environment taking pymetrozine as a unique carbon source and has very high pymetrozine degrading activity. The pymetrozine degrading bacterium IURM B56 can be applied to degradation of pymetrozine pesticides which pollutes the environment and has a very good application value in the aspects of soil pollution treatment and environmental remediation.

Description

Technical field[0001]The invention belongs to the biotechnology field, specifically a pyrid degradation bacterium Iurm B56 and its application thereof.Background technique[0002]my country is agricultural big country, with the development of socioeconomics and science and technology, people use a large number of pesticides to prevent pests and diseases, improve crops harvest, and increase economic benefits. Therefore, crops have increased year by year by year, while more than 1,500 pesticides used in my country, due to pyrid, the tattoolee-type mouthpiece of various crops (especially the lipuses, aphidic pests) It has been widely used in a well-prevention effect. However, as the long-term use of such pesticides, the environment is constantly accumulated in the environment, resulting in serious pollution of soil, water body and atmospheric environment, destroying the balance of the ecosystem, and also affects food safety and human livestock of agricultural products. Therefore, elimina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34A62D101/04A62D101/26A62D101/28C12R1/22C02F101/38C02F101/34
CPCA62D3/02C02F3/34A62D2101/04A62D2101/26A62D2101/28C02F2101/38C02F2101/34
Inventor 任建军李春雨张晋孙如梦王珍珠牛东泽朱劼车瑞兵张光民梁硕
Owner CHANGZHOU UNIV
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