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Mouse intestinal roll specimen preparation device and method

A technique for specimen preparation and mouse, applied in the preservation, application, animal husbandry, etc. of human or animal body, which can solve the intestinal injury, the operation of the longitudinal section and fixation method is difficult, and the transverse section and fixation method cannot completely present the intestine. It can reduce the pulling and pressing, shorten the production time, and the preparation process is convenient and quick.

Inactive Publication Date: 2021-04-02
李琳超
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a mouse intestinal roll sample preparation device and method, which solves the problem that the cross-section fixation method cannot completely present the intestine in the existing mouse intestinal tissue fixation method. The overall view of the tract tissue, the longitudinal section fixation method is difficult to operate and is likely to cause intestinal damage

Method used

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  • Mouse intestinal roll specimen preparation device and method
  • Mouse intestinal roll specimen preparation device and method

Examples

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Embodiment 1

[0059] Such as Figure 1~2 As shown, the mouse intestine roll specimen preparation device includes a winder, a wax strip 3 and a centering and rotating mechanism.

[0060] The winder includes a handle 1 and a deck 2 fixedly connected to one end of the handle 1. The handle 1 is a regular square prism rod. One end of the deck 2 is welded to one end of the handle 1, and the other end is provided with a jack. One end of the wax strip 3 is inserted in the jack of the card holder 2, and the other end protrudes from the jack of the deck 2, and the axis line of the wax strip 3 coincides with the axis line of the handle 1.

[0061] The centering rotary mechanism includes a bearing seat 41 , a socket 42 , a bearing 43 and an end cover 44 . The bearing housing 41 is provided with an inner cavity for installing the bearing, and an annular stepped surface is arranged in the inner cavity. The inserting sleeve 42 is cylindrical, and the center is provided with a through hole A penetrating ...

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Abstract

The invention discloses a mouse intestinal roll specimen preparation device and method, and relates to the technical field of animal specimen preparation. The mouse intestinal roll specimen preparation device comprises a winder and a wax strip, wherein the winder comprises a handle and a clamping base fixedly connected to one end of the handle, and an inserting hole is formed in the end, which isaway from the handle, of the clamping base; and one end of the wax strip is inserted into the inserting hole of the clamping base, and the other end of the wax strip extends out of the inserting holeof the clamping base. The mouse intestinal roll specimen preparation method is applied to the mouse intestinal roll specimen preparation device. The advantages are that 1, the Bouins liquid is used for carrying out pre-hardening treatment on the intestinal skin, so that the smoothness of the intestinal skin is favorably maintained, the pulling and pressing of the intestinal skin caused by the intestinal skin winding operation can be reduced, the operation difficulty during intestinal skin winding is reduced on the one hand, and the integrity of the intestinal morphological structure is guaranteed on the other hand; and 2, the preparation method of the intestinal roll is simple and easy to operate, consumes short time, can block endogenous or exogenous enzyme reaction in time, and is beneficial to ensuring the stability of protein in small intestine tissues;.

Description

technical field [0001] The invention relates to the technical field of animal specimen preparation, in particular to a device and method for preparing mouse intestinal roll specimens. Background technique [0002] The intestinal tract is an important organ for the transformation and absorption of organic matter, water, poisons, and drugs. Unlike other solid organ tissues, the intestinal tract presents a tubular cavity-like structure. The pathophysiological verification of intestinal tissue is an indispensable part of medical experimental research. In medical experiments, small animals such as rats, mice, and rabbits are usually used as the collection objects of intestinal samples. In the collection and fixation of samples, how to maintain the integrity of intestinal morphological structure and the stability of detection proteins has always been a technical problem that plagues researchers in the laboratory. [0003] Existing methods for fixing the intestinal tissues of expe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
CPCA01N1/00
Inventor 杨艺肖继
Owner 李琳超
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