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A kind of antibacterial composition containing Morinda officinalis extract and yellow sunflower extract

An extract, Morinda citrifolia technology, applied in the field of aquatic products, can solve the problems of delayed treatment, high cost, insignificant symptoms on the body surface, etc., and achieve long-term inhibitory effect and the effect of treating sarcoidosis

Active Publication Date: 2022-01-04
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The pathogens of fish visceral sarcoidosis are diverse, and some fishes are the infection hosts of several pathogens at the same time. The main pathogenic bacteria, mermaid photobacterium disease, pseudomonas disease and harvet vibriosis are the main diseases of large yellow croaker. Their symptoms are similar, which often lead to misdiagnosis and delayed treatment during the breeding process.
In addition, the symptoms of fish visceral sarcoidosis are not obvious in the early stage, and the diagnosis is very difficult. Once abnormal symptoms appear in the fish body, the disease has spread. Even if treatment measures are taken, the cost is often high and the desired effect may not be obtained. cause serious loss

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The preparation of embodiment 1 Morinda officinalis extract

[0024] Accurately weigh 1000g of Morinda officinalis, crush it and add 30 times the amount of sodium citrate buffer solution, then add 125μL / g of compound plant hydrolase, enzymolyze at 50°C for 2 hours, then sterilize in water bath, and filter with suction. Collect the enzymatic hydrolysis filtrate, then add 20 times of ethanol with a concentration of 60% to the filter residue, extract at 55°C for 2 hours, collect the extraction filtrate after suction filtration, and mix the enzymatic hydrolysis filtrate and the extraction filtrate Concentrate to obtain Morinda officinalis extract A.

[0025] Accurately weigh 1000g of Morinda officinalis, crush it and add 30 times the amount of sodium citrate buffer solution, then add 125μL / g of compound plant hydrolase, enzymolyze at 50°C for 2 hours, then sterilize in water bath, and filter with suction. Collect the enzymatic hydrolysis filtrate, then add 20 times of etha...

Embodiment 2

[0032] The antibacterial effect of embodiment 2 Morinda officinalis extract to Aeromonas schubert

[0033] 1. Preparation of culture medium

[0034] BHI medium was purchased from BD Company in the United States; 5% sheep blood agar plate, bacterial biochemical trace identification tube, etc. were purchased from Guangzhou Huankai Microbiology Co., Ltd.

[0035] 2. Preparation of bacterial suspension

[0036] Cultivation of test bacteria: activate the strain, streak on the solid medium with an inoculation needle, and culture at 28°C for 24 hours, pick a single colony, inoculate it on the nutrient broth medium and culture it with shaking for 24 hours. The bacterial solution was diluted to an appropriate concentration by plate counting method, and the concentration of the bacterial solution was 1×103 CFU / mL.

[0037] 3. Drug sensitivity test

[0038] The susceptibility test adopts the agar diffusion method, dilutes the bacterial suspension cultured to the logarithmic growth pha...

Embodiment 3

[0045] Embodiment 3 The influence of the ratio of Morinda officinalis extract and ambrette extract on antibacterial effect

[0046] Accurately weigh 1000g of Morinda officinalis, crush it and add 30 times the amount of sodium citrate buffer solution, then add 125μL / g of compound plant hydrolase, enzymolyze at 50°C for 2 hours, then sterilize in water bath, and filter with suction. Collect the enzymatic hydrolysis filtrate, then add 20 times of ethanol with a concentration of 60% to the filter residue, extract at 55°C for 2 hours, collect the extraction filtrate after suction filtration, and mix the enzymatic hydrolysis filtrate and the extraction filtrate Concentrate to obtain Morinda officinalis extract.

[0047] Accurately weigh 1000g of ambrette, dry it, pulverize and sieve it, add 20 times the amount of ethanol with a concentration of 70% to the obtained ambrette powder, ultrasonicate for 1.5 hours, repeat the extraction once, and mix the extracts to obtain amber sunflowe...

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PUM

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Abstract

The invention provides a bacteriostatic composition containing Morinda officinalis extract and ambrette extract, which is used in the preparation of sarcoidosis with Aeromonas schubert as the pathogen. application. The antibacterial composition containing Morinda officinalis extract and ambrette extract of the present invention has obvious inhibitory effect on Aeromonas schubert, especially has obvious and long-term inhibitory effect on snakehead fishes The composition can effectively treat the sarcoidosis of the snakehead fish, and the composition can be used for preparing medicaments and feed additives.

Description

technical field [0001] The invention belongs to the field of aquatic products, and in particular relates to an antibacterial composition containing Morinda officinalis extract and ambrette extract. Background technique [0002] my country is a big aquaculture country, and the aquaculture density and output of aquatic products are relatively high. However, due to backward aquaculture technology and facilities, fish bacterial diseases often occur frequently. At present, antibiotic-based drug control is still the most effective and direct method in aquaculture production, and it plays a very important role in the prevention and control of fish bacterial diseases. However, the long-term use of antibiotics in production practice will lead to the emergence of drug-resistant strains in a short period of time. The study found that during the eight years from 2009 to 2017, Aeromonas schubertii from the snakehead was resistant to tetracycline and aminoglycosides (such as kanamycin) a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/746A61P31/04A61K31/7036
CPCA61K31/7036A61K36/746A61K36/185A61P31/04A61K2236/19A61K2236/333A61K2236/51A61K2300/00
Inventor 刘春苏友禄李薇江飚马杰秦真东黄燕华林蠡
Owner ZHONGKAI UNIV OF AGRI & ENG
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