New method for utilizing substituted host to produce pine moth CPV pesticide

A qualitative polyhedron, virus insecticide technology, applied in biocides, plant growth regulators, botanical equipment and methods, etc., can solve the problems of workers' skin allergies, pine caterpillar poisoning, skin and flesh festering, etc. Achieve the effect of reducing production cost and high synchronization rate

Inactive Publication Date: 2003-10-29
环球生物农药有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is difficult to achieve industrialization due to high production costs, and the second method is suitable for the production of many viral insecticides
Yet there are two insurmountable obstacles in producing pine caterpillar virus with pine caterpillar, the one, the poisonous hair of pine caterpillar is extremely poisonous, and the artificial raising of pine caterpillar is easy to cause the skin allergy of worker, and severe case will cause skin and flesh to fester; Pine caterpillars are extremely selective for food. At present, there is no breakthrough in the technology of raising pine caterpillars with artificial feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Use 1×10 5 PIB / ml Wenshan pine caterpillar CPV suspension was sprayed to infect 3rd instar silver-leafed larvae on the artificial diet, collected in containers after the diseased larvae died, and stored in a 4°C refrigerator. When preparing insecticides, add an appropriate amount of phosphate buffer (PBS) mash the worm body, use 500rpm / min to pass 200 nylon yarn to remove the slag, count the polyhedron content in the suspension with the hemocytometer method, centrifuge the virus suspension after removing the slag, and then add emulsifiable oil to prepare the pine caterpillar CPV emulsion suspension Agent, light calcium carbonate or talcum powder can also be added, and wettable powder can be prepared by spray drying.

[0025] The pine caterpillar virus propagated by replacing the host has a killing effect of 88.5%, 82% and 75.6% on Wenshan pine caterpillar, Dechang pine caterpillar and masson pine caterpillar, respectively, according to the laboratory virulence test. Fo...

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PUM

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Abstract

The invention discloses a method for producing pine caterpillar polyhedrosis virus (CPV) insecticide. The method is to use silver printworm as a substitute host for the production of pine caterpillar polyhedrosis virus, and use artificial feed groups to raise silver Spodoptera moth, after being infected with pine caterpillar polyhedrosis virus, the sick dead insects are collected, and then processed to prepare a pine caterpillar polyhedrosis virus insecticide. The method adopts cheap artificial feed to carry out mass rearing of the silver print moth, which is convenient for feeding, can be produced in batches, and has low cost, and the obtained insecticide can be used for preventing and controlling Wenshan pine caterpillar, Dechang pine caterpillar and Masson pine caterpillar.

Description

(1) Technical field [0001] The present invention relates to a production method of a virus insecticide, specifically a new method for producing the pine caterpillar CPV insecticide by using a substitute host. (2) Background technology [0002] Cytopllasmic Polyhedrosis Viruses (CPV for short) is a natural pathogen of pine caterpillars. The pine caterpillar CPV insecticide has the characteristics of continuous and efficient control of pine caterpillar, safe and non-toxic to humans and animals. In 1974, the red pine caterpillar CPV was registered in Japan, becoming the first registered CPV insecticide in the world. In the early 1980s, my country began to isolate and study the pine caterpillar CPV pathogen. Through the national "Seventh Five-Year Plan" to "Ninth Five-Year Plan", it gradually developed to the current technology of using fences to multiply pine caterpillar virus to prepare insecticides at home and abroad. The fence production technology has different ages of inf...

Claims

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Application Information

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IPC IPC(8): A01N63/02
Inventor 孟小林姜小文徐进平叶林柏郜金荣钟铁柱
Owner 环球生物农药有限公司
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