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Sphingomonas and technology of sphingomonas to produce high-quality welan gum

The technology of sphingomonas and warm wheel glue is applied in the field of sphingomonas and its production of high-quality warm wheel glue, which can solve the problem that the temperature resistance of warm wheel glue is not outstanding, the dehydration is difficult, and the temperature resistance time is poor. It can improve the high temperature stability of product whiteness and viscosity, the strong ability of isopropanol to dissolve pigments, and the stable improvement of output and yield.

Active Publication Date: 2021-03-26
ORDOS ZHONGXUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the colloid yield produced by Alcaligenes currently in use is low, fluctuating at 1.0-1.5%, and the yield is unstable; the temperature resistance of the Wenlun rubber produced by the prior art is not outstanding, and the temperature resistance below 120 ° C is good. , 130 ℃ hot rolling for 4 hours, the viscosity will drop significantly, and 150 ℃ hot rolling for 4 hours, the viscosity will completely disappear, the temperature resistance time is too short under high temperature conditions, and the application is greatly restricted
At the same time, it is limited by the high viscosity of the Wenlun rubber fermentation liquid, which absorbs many impurities and is difficult to dehydrate.
The products produced by the existing production process have a dark brown appearance, a chroma of less than 40, a purity of less than 90%, high heavy metal content, many impurities, and poor microbial control of the finished product. They cannot be used as food additives in the food industry

Method used

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  • Sphingomonas and technology of sphingomonas to produce high-quality welan gum
  • Sphingomonas and technology of sphingomonas to produce high-quality welan gum
  • Sphingomonas and technology of sphingomonas to produce high-quality welan gum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Sphingomonas screening process:

[0030] 1. Material preparation

[0031] 1. Incline medium: 1.5% sucrose, 0.8% sodium chloride, 1.5% peptone, 0.3% beef extract, 0.65% yeast extract, 2.5% agar pH7.1;

[0032] 2. Fermentation medium: 2.5% glucose, 0.25% soybean protein powder, 0.05% NaCl, CaCO 3 0.2%, adjust the pH to 6-8.

[0033] 2. Breeding of strains

[0034] The original strains were subjected to plasma mutagenesis, and 50 strains were selected, and the selected strains were inoculated into the culture medium of the triangular flask, and after one, two, and three generations of subculture, the yield and quality indicators were analyzed, and the strain number DS320519 was found. Yield increased the most, product quality index improved significantly. DS320519 was subjected to multiple passage shake flask experiments, and the yield and quality indicators of the strain were stable.

[0035] For the evaluation of genetic stability, DS320519 was subjected to multipl...

Embodiment 2

[0039] A process for producing high-quality warm rubber, comprising the following steps:

[0040] (1) Preparation of fermentation strains: Sphingomonas DEOSEN-DS320519 stored at 4°C was inoculated into the seed medium, cultured at 30-32°C, and cultured for 24-36 hours.

[0041] The formula of 100ml seed medium: peptone 1g, glucose 0.5g, yeast powder 0.05g, salt 0.1g, calcium carbonate 0.02g, water 100g;

[0042] Fermentation medium: glucose 3.5-4.5%, soybean protein 0.8-1.0%, salt 0.01-0.05%, CaCO 3 0.1-0.3%, adjust the pH to 6.8-8.2.

[0043] (2) Liquid fermentation:

[0044] The seed liquid is cultivated at 30°C for 24-36 hours, then connected to the seed tank for expanded cultivation, the temperature is 30-32°C, the ventilation ratio is 0.9-1.0, and the cultivation is 36-48 hours; the fermentation conditions are: temperature 30-32°C; ventilation ratio 0- 0.2-0.3 in 24 hours, 0.4-0.5 in 24-48 hours, 0.7-1.0 after 48 hours; pH maintained at 6.8-8.2; fermentation time 65-72...

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Abstract

The invention discloses sphingomonas and a technology of the sphingomonas to produce high-quality welan gum. The sphingomonas is the sphingomonas DEOSEN-DS320519, deposited with the China General Microbiological Culture Collection Center and assigned the accession number CGMCC No. 19766; and the sphingomonas belongs to gram-negative bacteria and is stored on May 6th, 2020. After the fermentation of the sphingomonas DEOSEN-DS320519, the welan gum can be extracted from the fermentation product. Through the application of the strain and improved formula to carry out fermentation and production, the high temperature resistance of the prepared welan gum can be greatly enhanced when the temperature is 130-150 DEG C; the microbiological level of final products is less than 5000 cfu / g; the color changes from crineous color to pale yellow and then to off-white color; and the purity of the products can reach more than 95%, which exceeds microbial gum, xanthan gum and gellan gum for the same typeof food.

Description

technical field [0001] The invention relates to the technical field of sphingomonas and its application, in particular to a strain of sphingomonas and a process for producing high-quality Wenlun rubber. Background technique [0002] WELAN GUM is an industrial-grade microbial polysaccharide, a soluble exopolysaccharide produced by Alcaligenes sp through aerobic submerged fermentation. It has good thickening and anti-segregation properties, and is used in In the fields of cement mortar, concrete and other building materials, it can also be used in the oil drilling industry to provide thickening, suspension and rheological properties for oil mud, and can also be used in fine chemical, daily chemical and other industries. [0003] The production strain of Wenlun gum is inoculated in a sterile medium containing carbon source, nitrogen source, inorganic salt and water for aerobic fermentation, usually Alcaligenes is selected, and the fermentation product is extracted to obtain Wen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04
Inventor 孙正艳崔佳李延福吴尊德李瑞亭谢志新
Owner ORDOS ZHONGXUAN BIOCHEM
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