Porous polyurethane scaffold based on 3D printing

Abstract

The invention discloses a porous polyurethane scaffold based on 3D printing. The scaffold is formed by beta-TCP powder and polyurethane particles through a rapid forming technology. A method comprisesthe following steps of (1) building a polyurethane / beta-TCP additive manufacturing system; (2) preparing a polyurethane / beta-TCP scaffold printing consumable; and (3) performing 3D printing of the porous polyurethane scaffold. According to the scaffold material disclosed by the invention, a non-degradable material is used as an inner support material, so that cartilage tissues with more perfect forms can be constructed, and the requirement on the number of seed cells can be reduced.

Description

technical field [0001] The invention belongs to the technical field of 3D printing. The 3D printing technology is used to manufacture polyurethane / β-TCP novel semi-degradable composite auricle brackets in a personalized manner. Combined with stem cell technology, the auricular cartilage can be regenerated under the action of cartilage decellularized matrix. Background technique [0002] At present, the methods of clinical auricle repair include prosthesis repair (artificial ear) and surgical operation. Prosthetic restorations are simple, quick and inexpensive. However, there are problems such as poor simulation effect, material aging and discoloration, and difficult retention. There are two types of surgical implants: artificial prosthesis and autologous costal cartilage, and there has been controversy over these two surgical methods. The advantage of artificial prosthesis is that the implant is in a predetermined shape, which shortens the operation time; the main disadvan...

AI Technical Summary

Problems solved by technology

In the past, researchers constructed tissue-engineered cartilage with auricular shape in nude mice or through in vitro culture. For example, in the 1990s, the "human-eared mice" cultivated in foreign laboratories once caused a sensation in the world's biological and medical circles. , but it has obvious disadvantages: ①Using artificially synthesized absorbable exogenous scaffold materials, the degradation rate cannot be precisely controlled, and more or less deformation occurs as time goes by; ②The acidity of the scaffold materials Degradation products can easily lead to severe inflammatory reactions, and the animal model is a nude mouse animal model lacking normal immunity, lacking experimental data support for safe application in large animals with normal immunity

According to domestic and foreign literature reports, it is found that stem cells induced and differentiated in vitro are easy to form cartilage nodules in vitro, and it is difficult to separate them. The ability of cell proliferation and even apoptosis, coupled with the drastic change of the extracellular microenvironment after planting on the scaffold carrier, the cumbersome operation process increased the probability of cell contamination and many other reasons, make the effect of cartilage regeneration very unsatisfactory.