Enzyme-free pyruvic acid sensing detection method based on H2O2
A technology of pyruvate sensing and detection method, which is applied in the field of electrochemical sensing, can solve the problems of easy inactivation, difficult development of pyruvate biosensors, poor stability of natural enzymes, etc., and achieves a wide linear detection range, high efficiency and real-time Detection, long service life effect
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Embodiment 1
[0032]Example 1 Draw a standard curve
[0033](1) 50 mM PBS phosphate tendon (pH 6.5) is prepared as an electrode buffer;
[0034](2) Constructing a three-electrode system, wherein the working electrode is a Pusher blue repair electrode, and the electrolyte solution is 50ml of electrode buffer, and the applied potential is -0.05V, performs timing current scan, activates 30 min, to the current baseline stable, record the corresponding current Value i0;
[0035](3) Add H in electrode buffer2O2, Make it a final concentration of 0.2 mm, and the current value I is recorded after the current value is stable.1;
[0036](4) Apxtone is added to the electrode buffer and the sodium pyruvate of different concentration gradients, so that the final concentration is 0, 1, 2, 4, 6, 8 and 10 mm, record current changes, and accurately recorded Current value I when pyruvate solution 25S, 50S, 100S, and 150S2. Such asfigure 1 As shown, after adding pyruvate sodium, the current value has slowly decreases, that is, ...
Embodiment 2
[0038]Example 2 actual application
[0039]Apyclhancetate is added to acetate in acetate, pneumoniae, and acetate, acetate, respectively, a pyreonate, respectively, an exogenous amount of acetate, and a preparation method provided in accordance with the detection method provided in accordance with the present invention. Perform test, at 25 ml of 0.2mm h2O21 ml of the sample to be tested is added to the electrode buffer, and the current change in real-time recording of the Prussian blue dressing electrode. Sodium pyruvate concentration in sample*= (25.27 × W-0.32) × 26.
[0040](1) Clostridium Acetobutylicum at a 40 ml fermentation system for anaerobic fermentation of anaerobic fermentation, the main product of the fermentation broth was detected by the high efficiency gas chromatograph, which was 12.1 g / L butanol, 6.3 g / L. Taric acid, 2.0 g / l ethanol, and 1.6 g / L acetone, sodium pyruvate having a final concentration of 11.0 g / L, for sample 1.
[0041](2) Klebsiella pneumoniae in 40...
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