Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Composition, sampling device, kit and home virus detection method

A sampling device and composition technology, applied in the field of detection, can solve problems such as inability to use nasopharyngeal swab samples for preservation, and inability to use nucleic acid detection, etc.

Pending Publication Date: 2021-03-19
BIOCHAIN BEIJING SCI & TECH
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used virus inactivators such as formaldehyde can denature proteins through cross-linking, and at the same time react with amino groups of nucleotides. The inactivation effect is good, but it cannot be used for the preservation of nasopharyngeal swab samples, and cannot be used for follow-up nucleic acid detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition, sampling device, kit and home virus detection method
  • Composition, sampling device, kit and home virus detection method
  • Composition, sampling device, kit and home virus detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0049] (1) Preparation of compositions for sample preservation

[0050] Prepare Hanks Balanced Salt Solution: stock solution A: NaCl 160g, MgSO 4 ·7H 2 O 2g, KCl 8g MgC l2 ·6H 2 O2g CaCl 2 2.8g was dissolved in 1000ml double distilled water; stock solution B: (1)Na 2 HPO 4 12H 2 O 3.04g, KH 2 PO 4 1.2g, 20.0g glucose dissolved in 800ml double distilled water, (2) 0.4% phenol red solution: take 0.4g phenol red, put it in a glass mortar, add 0.1N NaOH drop by drop, and grind until completely dissolved, add about 0.1 N NaOH 10ml. Inhale the dissolved phenol red into a 100ml measuring bottle, wash the remaining phenol red liquid in the mortar with double distilled water, put it into the measuring bottle, and finally add double distilled water to 100ml. Mix liquid (1) and liquid (2), and add double distilled water to 1000ml, which is stock solution B. Application solution: 1 part stock solution A, 1 part stock solution B, 18 parts double distilled water.

[0051] Prep...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composition for sample preservation. The composition comprises penicillin, antifungal element, guanidinium isothiocyanate and lithium chloride, wherein the penicillin, the antifungal element, the guanidinium isothiocyanate and the lithium chloride are dissolved in a Hanks equilibrium salt solution. According to the composition disclosed by the invention, by optimizing liquid components in the composition, a sample can be preserved at normal temperature, and meanwhile, on the premise of ensuring virus inactivation, the composition can be used for subsequent protein immunodetection and nucleic acid detection. Meanwhile, due to the normal-temperature storage capability, the household sampling of a patient can be facilitated.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a composition, a sampling device, a kit and a home virus detection method. Background technique [0002] Virus is a kind of non-cellular organism with small size, simple structure and only one kind of nucleic acid (DNA or RNA), which must parasitize and multiply in living cells. A virus is a non-cellular life form, which is composed of a long chain of nucleic acid and a protein shell. The virus has no metabolic mechanism of its own, and no enzyme system. Therefore, when the virus leaves the host cell, it becomes a chemical substance without any life activities and cannot reproduce itself independently. Once it enters the host cell, it can use the material and energy in the cell and the ability of replication, transcription and translation to produce a new generation of viruses like it according to the genetic information contained in its own nucleic acid. There ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/24C12M1/30C12Q1/70C12Q1/6806G01N1/28
CPCC12Q1/24C12M33/02C12Q1/701C12Q1/6806G01N1/28C12Q2527/125
Inventor 韩晓亮吴振李永君王建铭
Owner BIOCHAIN BEIJING SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com