Marker of pathogenic gene of hereditary thyroid dysfunction disease and detection kit thereof
A technology of thyroid function and detection kit, which is applied in the field of biomedicine and can solve the problems of large sample demand, small throughput, and low sensitivity
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Embodiment 1
[0024] 1.1 Specimen collection and storage
[0025] 1.1.1 Blood samples
[0026] All blood samples were collected in special tubes, among which, common tubes used for biochemical assays were used for serum collection, and EDTA anticoagulant tubes were used for plasma collection. Anticoagulant blood samples were collected and stored in a -80°C ultra-low temperature refrigerator.
[0027] 1.1.2 The general steps of peripheral blood DNA extraction and storage (according to the QIAamp DNA Mini Kit instructions) are as follows:
[0028] (1) Cleavage and digestion (2) DNA adsorption (3) DNA washing (4) DNA elution (5) DNA concentration determination and quality assessment: use NanoDrop spectrophotometer to measure DNA absorbance and concentration, the concentration is 20-200ng / μL , OD260 / OD280 ratio is in the range of 1.8-2.0; DNA integrity is detected by agarose gel electrophoresis, and the general DNA fragment size is 20-40kb. (6) Sample storage: The obtained peripheral blood DNA...
Embodiment 2
[0210] Clinical Application of Thyroid Platform
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