Soil fumigation product taking cinnamyl aldehyde as active component and application of soil fumigation product
A technology of cinnamaldehyde and fumigant, applied in the field of pesticides
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Embodiment 2
[0120] Embodiment 2 is to underground insect pest toxicity test
[0121] Root-knot nematode: refer to NY / T 1833.1-2009 "Pesticide Indoor Bioassay Test Guidelines for Nematicides" Part 1: Inhibition of plant pathogenic nematodes test by insect dipping method. On the basis of pre-testing to determine the concentration range of the agent, dilute cinnamaldehyde (1% acetone solution) with clear water to 5 to 7 serial concentrations, and use an aqueous solution containing Tween 80 emulsifier and acetone (wherein the volume ratio of acetone and water 5:95) (Tween 80+diluted 100 times with acetone) as a control. Prepare the collected second-instar larvae into a suspension (about 100 pieces / mL) with clean water, pipette 0.5mL of the drug solution and 0.5mL of the second-instar larvae suspension into a 2mL counting dish, repeat 3 times, and use Thin glass rods were mixed and placed in a constant temperature incubator at 25°C. After 48 hours, the number of dead and alive nematodes was c...
Embodiment 3
[0128] Embodiment 3 control soil-borne disease test
[0129] Mycelium growth rate method: This test is determined by the mycelium growth rate method in accordance with the Agricultural Industry Standard of the People's Republic of China (NY / T1156.2-2006). The medicament (cinnamaldehyde) was diluted with sterile water into a series of concentration gradient mother solutions (25mg / L, 50mg / L, 100mg / L, 150mg / L, 200mg / L). Under aseptic operation conditions, according to the test treatment, quantitatively add the pre-thawed culture medium into the sterile conical flask, quantitatively absorb the liquid medicine from low concentration to high concentration, add them respectively to the above conical flasks, and shake well. Then pour the same amount into three petri dishes with a diameter of 9 cm to make a PDA plate containing the drug at the corresponding concentration; the cultured pathogenic bacteria were punched from the edge of the colony with a sterilized puncher with a diameter...
Embodiment 4
[0134] Embodiment 4 field efficacy test
[0135] Test drug:
[0136] Table 4 Reagent table
[0137]
[0138]
[0139] 5% cinnamaldehyde EC: 5% cinnamaldehyde, 2% 2,6-di-tert-butyl-4-methylphenol (BHT), 8% Tween 80, and cyclohexanone.
[0140] 5% cinnamaldehyde emulsion in water: 5% cinnamaldehyde, 2% 2,6-di-tert-butyl-4-methylphenol (BHT), 8% Tween 80, 20% cyclohexanone, and water.
[0141] 5% cinnamaldehyde granules: 5% cinnamaldehyde, 2% 2,6-di-tert-butyl-4-methylphenol (BHT), 2% DMSO, 5% NNO, filled with bricks.
[0142] The formulations involving 5% cinnamaldehyde emulsifiable concentrate, 5% cinnamaldehyde water emulsion and 5% cinnamaldehyde granules in the following examples are the same as here.
[0143] Test crop: cucumber
[0144] Control object: root-knot nematode
[0145] Dosage of active ingredients: 1.5 kg / ha
[0146] Disease index survey and preventive effect survey methods:
[0147] Crush the diseased roots of cucumber root knots, mix them with 9 t...
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